缺血预处理对糖尿病大鼠心肌缺血再灌注损伤AMPK通路的影响  

Effect of ischemic preconditioning on myocardial ischemia reperfusion injury and AMPK signal pathway in diabetic rats

作　　者：谢映红[1] 王巍[2] 

机构地区：[1]解放军第202医院综合科,辽宁沈阳110003 [2]解放军第202医院内分泌科,辽宁沈阳110003

出　　处：《实用临床医药杂志》2016年第7期6-9,共4页Journal of Clinical Medicine in Practice

基　　金：辽宁省自然科学基金(2013020220)

摘　　要：目的探讨缺血预处理对糖尿病大鼠心肌缺血再灌注损伤的保护作用。方法 24只SD大鼠随机分为4组,即糖尿病缺血再灌注组(DMI/R组)、非糖尿病缺血再灌注组(NDMI/R组)、糖尿病缺血预处理组(DMIPC组)及非糖尿病缺血预处理组(NDMIPC组)。应用2%链脲佐菌素腹腔注射方法建立糖尿病大鼠模型,造模后第10周建立离体心脏灌注模型。检测各组大鼠复灌后冠脉流出液肌酸激酶含量、心肌含水率变化及心排血量、左心室发展压(LVDP)、左心室内压最大上升和下降速率的恢复率,并利用Western blot方法检测心肌组织腺苷酸活化蛋白激酶(AMPK)及磷酸化AMPK蛋白表达水平。结果糖尿病大鼠复灌后冠脉肌酸激酶含量及心肌含水率显著高于非糖尿病组大鼠(P<0.05),左室功能恢复率显著低于非糖尿病组(P<0.05),AMPK及磷酸化AMPK表达水平均显著低于非糖尿病组(P<0.05)。糖尿病大鼠缺血预处理组与缺血再灌注组上述各指标比较无显著差异(P>0.05)。结论心肌缺血预处理对糖尿病大鼠心肌缺血再灌注损伤的保护作用不明显,可能与糖尿病大鼠心肌AMPK信号通路受抑有关。Objective To explore the effect of myocardial ischemic preconditioning（ MIPC）on myocardial ischemia reperfusion injury（ MI / R） in diabetic rats. Methods A total of 24 SD rats were divided into diabetic rats with myocardial ischemia reperfusion injury group（ DMI / R group）,diabetic rats with ischemic preconditioning group（ DMIPC group）,non-diabetic rats with myocardial ischemia reperfusion injury group（ NDMI / R group） and non-diabetic rats with ischemic preconditioning group（ NDMIPC group）. 2% streptozotocin was used to induce diabetes mellitus in rats. The isolated heart perfusion Langendorff models were established at tenth week. Creatine kinase content of coronary outflow fluid and myocardial water content were measured. The cardiac output（ CO）,left ventricular developed pressure（ LVDP）,the maximum upstroke and decay velocities of left ventricular pressure were recorded. The protein expression level of AMPK and p AMPK were detected by Western blot analysis. Results Creatine kinase content of coronary outflow fluid and myocardial water content after re-perfusion in diabetic rats were significantly higher than those in the non-diabetic rats groups（ P〈0. 05）. Left ventricular function recovery rate in diabetic groups was significantly lower than that in the non-diabetic groups（ P〈0. 05）. The expression levels of AMPK and p AMPK in diabetic rats were significantly lower than those in non-diabetic rats（ P〈0. 05）. However,no significant differences in the indexes mentioned above were observed between DMI / R and DMIPC group（ P〈0. 05）. Conclusion Theprotectiveeffectofmyocardialischemicpreconditioningonmyocardial ischemia reperfusion injury in diabetic rats is not obvious,which may be related to the inhibition of AMPK signaling pathway in the myocardium of diabetic rats.

关 键 词：缺血预处理 糖尿病 心肌缺血再灌注损伤 腺苷酸活化蛋白激酶 

分 类 号：R587.1[医药卫生—内分泌]