不同病程的慢性乙型肝炎病毒感染者外周血中调节性T细胞和自然杀伤细胞的表达及相关性  

Expression of regulatory T cells and natural killer cells in peripheral blood of patients in different stages of chronic hepatitis B virus infection

作　　者：张晓慧[1] 娄金丽[2] 段钟平[1] 陈煜[1] 

机构地区：[1]首都医科大学附属北京佑安医院人工肝中心,北京市100069 [2]首都医科大学附属北京佑安医院临检中心,北京市100069

出　　处：《世界华人消化杂志》2016年第12期1885-1890,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81500472;北京市医院管理局临床医学发展专项基金资助项目;No.XM201308;北京市医院管理局"登峰"人才培养计划基金资助项目;No.DFL20151601;国家临床重点专科建设资助项目;No.WJWYA-2014-002;首都医科大学基础-临床合作基金资助项目;Nos.14JL73;14JL72~~

摘　　要：目的:观察乙型肝炎病毒(hepatitis B virus,HBV)慢性感染者外周血中调节性T细胞(regulatory T cells,Tregs)和自然杀伤(natural killer,NK)细胞的表达及相关性.方法:选取HBV慢性感染患者共96例,其中慢性乙型肝炎患者42例,乙型肝炎肝硬化患者30例,HBV携带者24例,抽取外周血用流式细胞分析技术检测其Tregs和NK细胞的表达情况.同时选取健康者21例作为对照组.结果:HBV慢性感染者外周血CD4+CD25+Foxp3+Tregs占CD4+T细胞的比例整体高于健康对照组,其中乙型肝炎肝硬化患者外周血CD4+CD25+Foxp3+Tregs占CD4+T细胞的比例最高,其次为慢性乙型肝炎患者,最后为HBV携带者和健康对照组.相反,NK细胞占淋巴细胞的比例在HBV慢性感染者外周血中整体较健康对照组低,其中乙型肝炎肝硬化组表达最低,其次为慢性乙型肝炎组,最后为HBV携带者,但都低于健康对照组.进一步分析显示NK细胞的表达与Tregs呈负相关性(r=-0.3280,P<0.05).结论:不同阶段的HBV慢性感染患者外周血中Tregs和NK细胞表达不同,且两者呈明显的负相关性.AIM：To investigate the expression of regulatory T cells（Tregs） and natural killer（NK） cells in the peripheral blood of patients with chronic hepatitis B virus（HBV） infection.METHODS：A total of 96 chronic HBV infected patients were enrolled,including 42 chronic hepatitis B patients,30 cirrhosis patients,and24 hepatitis B carriers.There were 21 healthy persons as controls.Peripheral blood was collected from subjects to detect the expression of Tregs and NK cells,by means of flow cytometry.RESULTS：The expression of CD4＋CD25＋Foxp3＋Tregs in peripheral blood was the highest inthe hepatitis B cirrhosis group,followed by chronic hepatitis B patients,HBV carriers and healthy controls.In contrast,the percentage of NK cells in the cirrhosis group was the lowest,followed by chronic hepatitis B patients and HBV carriers,but all of them had lower percentage of NK cells than healthy controls.Further analysis showed that the expression of Tregs was negatively correlated with NK cells（r=-0.3280,P 〈0.05）.CONCLUSION：The expression of Tregs and NK cells in the peripheral blood of chronic HBV infected patients is different with the disease course,and they are negatively correlated.

关 键 词：调节性T细胞 自然杀伤细胞 乙型肝炎病毒慢性感染 

分 类 号：R512.62[医药卫生—内科学]