Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus  

作　　者：纪利芹 蒋克勇 刘梅 王宝杰 韩龙江 张明明 王雷 

机构地区：[1]Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences, Qingdao 266071, China [2]University of Chinese Academy of Sciences, Beijing 100049, China [3]Fisheries College, Ocean University of China, Qingdao 266003, China

出　　处：《Chinese Journal of Oceanology and Limnology》2016年第3期430-440,共11页中国海洋湖沼学报（英文版）

基　　金：Supported by the Special Foundation for Youth Science and Technology of Qingdao(No.14-2-4-59-jch);the Science and Technology Development Planning of Shandong Province(No.2011GGC21007);the National Natural Science Foundation of China(No.41206144)

摘　　要：To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot （Seophthalmus maximus）, water temperature was lowered rapidly from 18 to 1℃. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1℃. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly （P〈0.05） as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1℃ and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8℃, and creatinine concentration was highest at 3℃. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly （P〈0.05） as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5℃ may induce a stress response in S. maximus and that temperature should be kept above 8℃ in the aquaculture setting to avoid damage to the fish.To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot( Scophthalmus maximus),water temperature was lowered rapidly from 18 to 1°C.During the cooling process,three individuals were removed from culture tanks at 18,13,8,5,3,and 1°C.Blood samples and tissues were taken from each individual,hematological indices and HSP gene expression in tissues were measured.The red blood cell count,white blood cell count,and hemoglobin concentration decreased significantly( P < 0.05) as temperature decreased.Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased,whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1°C and lactate dehydrogenase activity increased first and then decreased.Blood urea nitrogen and uric acid levels were highest at 8°C,and creatinine concentration was highest at 3°C.The concentrations of plasma cortisol,cholesterol,and triglyceride all increased significantly( P < 0.05) as temperature decreased.The serum glucose concentration increased first and then decreased to the initial level.The HSP70 m RNA expression showed various patterns in different tissues,whereas HSP90 m RNA expression showed the same tendency in all tissues.Overall,these results indicate that temperature decreases in the range of 8 to 5°C may induce a stress response in S.maximus and that temperature should be kept above 8°C in the aquaculture setting to avoid damage to the fish.

关 键 词：HEMATOLOGY HSP70 HSP90 mRNA expression low temperature stress fish 

分 类 号：S966.5[农业科学—水产养殖] Q78[农业科学—水产科学]