Significant population genetic structure detected in the rock bream Oplegnathus fasciatus(Temminck & Schlegel,1844) inferred from fluorescent-AFLP analysis  

作　　者：肖永双 马道远 徐世宏 刘清华 王彦丰 肖志忠 李军 

机构地区：[1]Key Laboratory of Experimental Marine Biology , Institute of Oceanology , Chinese Academy of Sciences [2]Nantong Branch , Institute of Oceanology , Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2016年第3期441-450,共10页中国海洋湖沼学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A413-1);the STS(No.KFJ-EW-STS-060-STS);the Natural Science Foundation of Jiangsu Province(No.BK2012222);the Key Laboratory of East China Sea&Oceanic Fishery Resources Exploitation and Utilization,Ministry of Agriculture,China(No.K201204);the Promotive Research Fund for Excellent Young and Middle-Aged Scientists of Shandong Province(No.BS2012HZ025);the National Natural Science Foundation of China(No.41506170);the Technology Foundation for Selected Overseas Chinese Scholar;Ministry of Personnel of China(No.2013.01)

摘　　要：Oplegnathusfasciatus （rock bream） is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplifed fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1 264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei＇s genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance （AMOVA） showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a significant population genetic structure existed in the species. The pairwise fixation index Fst ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O.fasciatus populations examined should comprise two stocks.Oplegnathus fasciatus(rock bream) is a commercial rocky reef fish species in East Asia that has been considered for aquaculture.We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplified fragment length polymorphisms technology.Using 53 individuals from three populations and four pairs of selective primers,we amplified 1 264 bands,98.73% of which were polymorphic.The Zhoushan population showed the highest Nei's genetic diversity and Shannon genetic diversity.The results of analysis of molecular variance(AMOVA) showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations,which indicated that a significant population genetic structure existed in the species.The pairwise fixation index F_(st) ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction.The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China.The AMOVA and STRUCTURE analyses suggested that the O.fasciatus populations examined should comprise two stocks.

关 键 词：Oplegnathusfasciatus fluorescent-AFLP genetic diversity genetic struc^a＇e 

分 类 号：S917.4[农业科学—水产科学]