Identification and bioinformatics analysis of micro RNAs from the sporophyte and gametophyte of Pyropia haitanensis  

作　　者：黄爱优 王广策 

机构地区：[1]Institute of Oceanology , Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2016年第3期451-459,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A406);the National Natural Science Foundation of China(Nos.41176134,41306151);the China Strategic Leading Special Science and Technology Academy(No.XDA11020404)

摘　　要：Pyropia haitanensis(T.J.Chang et B.F.Zheng) N.Kikuchi et M.Miyata( Porphyra haitanensis) is an economically important genus that is cultured widely in China.P.haitanensis is cultured on a larger scale than Pyropia yezoensis,making up an important part of the total production of cultivated Pyropia in China.However,the majority of molecular mechanisms underlying the physiological processes of P.haitanensis remain unknown.P.haitanensis could utilize inorganic carbon and the sporophytes of P.haitanensis might possess a PCK-type C 4-like carbon-fixation pathway.To identify micro RNAs and their probable roles in sporophyte and gametophyte development,we constructed and sequenced small RNA libraries from sporophytes and gametophytes of P.haitanensis.Five micro RNAs were identified that shared no sequence homology with known micro RNAs.Our results indicated that P.haitanensis might posses a complex s RNA processing system in which the novel micro RNAs act as important regulators of the development of different generations of P.haitanensis.Pyropia haitanensis （T. J. Chang et B. F. Zheng） N. Kikuchi et M. Miyata （Porphyra haitanensis） is an economically important genus that is cultured widely in China. P. haitanensis is cultured on a larger scale than Pyropia yezoensis, making up an important part of the total production of cultivated Pyropia in China. However, the majority of molecular mechanisms underlying the physiological processes of P. haitanensis remain unknown. P. haitanensis could utilize inorganic carbon and the sporophytes of P. haitanensis might possess a PCK-type C4-like carbon-fixation pathway. To identify microRNAs and their probable roles in sporophyte and gametophyte development, we constructed and sequenced small RNA libraries from sporophytes and gametophytes ofP. haitanensis. Five microRNAs were identified that shared no sequence homology with known microRNAs. Our results indicated that P. haitanensis might posses a complex sRNA processing system in which the novel microRNAs act as important regulators of the development of different generations of P. haitanensis.

关 键 词：MICRORNA Pyropia haitanensis small RNA SPOROPHYTE GAMETOPHYTE 

分 类 号：S917.3[农业科学—水产科学]