Growth and antioxidant status of oriental river prawn Macrobrachium nipponense fed with diets containing vitamin E  

作　　者：赵卫红 王资生 於叶兵 齐志涛 吕林兰 张余霞 吕富 

机构地区：[1]School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China [2]Jiangsu Provincial Key Laboratory of Coastal Pond Aquaculture Ecology, Yancheng 224051, China

出　　处：《Chinese Journal of Oceanology and Limnology》2016年第3期477-483,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.31101887);the Natural Science Foundation of Jiangsu Province(Nos.BK2011419,BK2012675);the Special Projects in Northern Jiangsu Province(No.BN2015107);the Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection foundation(No.JLCBE07009)

摘　　要：A feeding trial was carried out to investigate the dietary vitamin E requirement of the oriental river prawn Macrobrachium nipponense(weight of 0.3–0.4 g) and its effect role on antioxidant activity.Prawns were fed with seven levels of vitamin E(0,25,50,75,100,200,and 400 mg/kg diet) for 60 days.The results show that dietary vitamin E supplementation could significantly increased the prawn weight( P <0.05).The activity of superoxide dismutase(SOD) in the hepatopancreas was significantly higher in prawns fed with diets supplemented with ≤75 mg/kg vitamin E than in those fed with diets supplemented with 100–400 mg/kg vitamin E( P <0.05).The activity of catalase(CAT) in the hepatopancreas decreased significantly as dietary vitamin E supplementation increased( P <0.05),and no significant difference was detected in glutathione peroxidase(GSH-Px) activity between different dietary groups( P >0.05).The contents of vitamin E in the hepatopancreas and in the muscle increased with increasing dietary vitamin E.There was a linear correlation between the vitamin E level in diet and that in muscle,and between the vitamin E level in diet and that in the hepatopancreas.All the above results indicated that dietary vitamin E can be stored in the hepatopancreas and muscle and lower both the activities of SOD and CAT in the hepatopancreas,suggesting that it is a potential antioxidant in M.nipponense.Broken line analysis conducted on the weight gains of prawns in each diet group showed that the dietary vitamin E requirement for maximum growth is 94.10 mg/kg.A feeding trial was carried out to investigate the dietary vitamin E requirement of the oriental river prawn Macrobrachium nipponense （weight of 0.3-0.4 g） and its effect role on antioxidant activity. Prawns were fed with seven levels of vitamin E （0, 25, 50, 75, 100, 200, and 400 mg/kg diet） for 60 days. The results show that dietary vitamin E supplementation could significantly increased the prawn weight （P〈0.05）. The activity of superoxide dismutase （SOD） in the hepatopancreas was significantly higher in prawns fed with diets supplemented with ≤75 mg/kg vitamin E than in those fed with diets supplemented with 100-400 mg/kg vitamin E （P〈0.05）. The activity of catalase （CAT） in the hepatopancreas decreased significantly as dietary vitamin E supplementation increased （P〈0.05）, and no significant difference was detected in glutathione peroxidase （GSH-Px） activity between different dietary groups （P〉0.05）. The contents of vitamin E in the hepatopancreas and in the muscle increased with increasing dietary vitamin E. There was a linear correlation between the vitamin E level in diet and that in muscle, and between the vitamin E level in diet and that in the hepatopancreas. All the above results indicated that dietary vitamin E can be stored in the hepatopancreas and muscle and lower both the activities of SOD and CAT in the hepatopancreas, suggesting that it is a potential antioxidant in M. nipponense. Broken line analysis conducted on the weight gains of prawns in each diet group showed that the dietary vitamin E requirement for maximum growth is 94.10 mg/kg.

关 键 词：Macrobrachium nipponense vitamin E weight gain superoxide dismutase （SOD） catalase （CAT） glutathione peroxidase （GSH-Px） 

分 类 号：S966.12[农业科学—水产养殖]