Integration of Chemical Methods and Biomarkers for Assessment of Chlorimuron-Ethyl Bioavailability in Soil  

作　　者：REN Wenjie WANG Meie ZHOU Qixing TENG Ying 

机构地区：[1]Key Laboratory of Soil Environment and Pollution Remediation of Chinese Academy of Sciences,Institute of Soil Science,Chinese Academy of Sciences [2]Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology,Chinese Academy of Sciences [3]State Key Laboratory of Urban and Regional Ecology,Research Centre for Eco-Environmental Sciences,Chinese Academy of Sciences [4]Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education),College of Environmental Science and Engineering,Nankai University

出　　处：《Pedosphere》2016年第3期273-281,共9页土壤圈（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.41401565,41201520 and 20807046)

摘　　要：Bioavailability is a critical factor for assessing the environmental risk of organic pollutants in soil. In this study, extractions with 3 different solvents, including 2 aqueous solutions, calcium chloride(CaCl_2) and a phosphate buffer solution(PBS), and a mixture of aqueous solution and organic solvent, a PBS-methanol(8:2,volume/volume) mixture(PBS-M), were performed to assess the bioavailability of chlorimuron-ethyl in soil in comparison to a battery of toxicity tests in wheat seedlings. The results indicated that the peroxidase(POD) activity in wheat leaves after 7 d of exposure was one of the sensitive biomarkers of chlorimuron-ethyl in soil.The extractability of chlorimuron-ethyl by all the 3 solvents decreased with exposure time, and the rate of decrease of the PBS-M extraction between 1 and 7 d of exposure was substantially higher than those of the aqueous solution extractions. Chlorimuron-ethyl gradually changed from a water-soluble form into a soil organic matter(SOM)-bound form in the soil. The PBS extraction correlated best with the POD activity in the leaves after 7 d of exposure.Bioavailability is a critical factor for assessing the environmental risk of organic pollutants in soil. In this study, extractions with 3 different solvents, including 2 aqueous solutions, calcium chloride（CaCl2） and a phosphate buffer solution（PBS）, and a mixture of aqueous solution and organic solvent, a PBS-methanol（8：2,volume/volume） mixture（PBS-M）, were performed to assess the bioavailability of chlorimuron-ethyl in soil in comparison to a battery of toxicity tests in wheat seedlings. The results indicated that the peroxidase（POD） activity in wheat leaves after 7 d of exposure was one of the sensitive biomarkers of chlorimuron-ethyl in soil.The extractability of chlorimuron-ethyl by all the 3 solvents decreased with exposure time, and the rate of decrease of the PBS-M extraction between 1 and 7 d of exposure was substantially higher than those of the aqueous solution extractions. Chlorimuron-ethyl gradually changed from a water-soluble form into a soil organic matter（SOM）-bound form in the soil. The PBS extraction correlated best with the POD activity in the leaves after 7 d of exposure.

关 键 词：chemical extraction environmental risk organic pollutants organic solvent peroxidase activity phosphate buffer solution toxicity test 

分 类 号：X825[环境科学与工程—环境工程]