不同地区广金钱草遗传多样性的ISSR分析  

Genetic Diversity Analysis of Desmodium styracifolium from Different Habitats by ISSR Analysis

作　　者：何博[1] 许勇 杨旻[1] 陈科力[1] 黄必胜[1] 

机构地区：[1]教育部中药资源与中药复方重点实验室&湖北中医药大学,武汉430065 [2]武汉人福医药集团医药研究院

出　　处：《中国药师》2016年第5期884-887,共4页China Pharmacist

基　　金：国家科技重大专项(编号:2014ZX09304307001)

摘　　要：目的:对广东省、广西壮族自治区共15个产地的广金钱草遗传多样性以及亲缘性关系进行分析。方法:应用inter-simple sequence repeat(ISSR)技术,对广东省、广西壮族自治区的样品进行遗传多样性分析;使用Popgene 1.32软件对扩增结果进行分析,并使用UPGMA软件绘制聚类图。结果:7条ISSR引物扩增出的产物共有51条清晰的条带,其中多样性条带为41条;Shannon多样性指数(I)为0.3,Nei’s遗传多样性指数(H)为0.246 4,居群间的遗传分化系数(G_(ST))为0.123 8,基因流(N_m)为3.539 7。结论:广东、广西两地栽培以及野生广金钱草具有较高的遗传多样性,其遗传距离跟地理距离有一定的关联性。Objective：To assess the genetic diversity and phylogenetic relationship of Desmodium styracifolium from fifteen regions of Guangdong and Guangxi Zhuang autonomous region.Methods：The molecular technique ISSR（（inter-simple sequence repeat）） was applied to investigate the genetic diversity of Desmodium styracifolium from fifteen regions of Guangdong and Guangxi Zhuang autonomous region.The data was analyzed with Popgene 1.32,and a cluster diagram was presented by UPGMA.Results：Totally 51 amplified fragments were obtained by 7 ISSR primers.The results analyzed by Popgene 1.32 showed that the Shannon diversity index（I） was 0.3,the NEI＇s genetic diversity coefficient（H） was 0.246 4,the coefficient of genetic differentiation（GST） was 0.123 8,and the gene flow（Nm） was 3.539 7.Conclusion：The above mentioned results exhibit that Desmodium styracifolium from Guangdong,Guangxi and some wild herbal populations has high genetic diversity.The clustering results illustrate that the genetic distance of Desmodium styracifolium originated from Guangdong and Guangxi is related with geographic distance.

关 键 词：广金钱草 ISSR技术 遗传多样性 

分 类 号：Q943[生物学—植物学]