GC法测定丙酮酸乙酯的含量  

Determination of Ethyl Pyruvate by GC

作　　者：王晓云[1] 毛发燕 王小刚[2] 黄雁茹 海春旭[3] 刘鹏[1] 何炜[1] 

机构地区：[1]第四军医大学药学系化学教研室,西安710032 [2]第四军医大学学员旅五营十九连,西安710032 [3]第四军医大学预防医学系毒理学教研室,西安710032

出　　处：《中国药师》2016年第5期1021-1023,共3页China Pharmacist

基　　金：陕西省科技统筹计划重点项目(编号:2012KTCQ0302)

摘　　要：目的:建立测定丙酮酸乙酯原料含量的气相色谱法。方法:色谱柱:Varian CP7502毛细管柱(25 m×0.25 mm,0.25μm);载气:氮气,流量为30 ml·min^(-1);燃气:氢气,流量为40 ml·min^(-1);助燃气:空气,流量为400 ml·min^(-1);检测器:氢火焰离子化检测器(FID);进样口温度:210℃;升温程序:初始温度为40℃,以15℃·min^(-1)的速度升至200℃;分流比:100∶1;进样量:1μl。结果:丙酮酸乙酯进样浓度在0.025 6~8.192 8 mg·ml^(-1)范围内与内标物的峰面积比值呈良好的线性关系(r=0.999 8),平均加样回收率为99.99%,RSD为1.38%(n=9)。结论:建立的GC法准确、简便、快速、稳定性好,检测灵敏度高,适用于丙酮酸乙酯原料药的含量测定。Objective：To establish a method for the determination of ethyl pyruvate by GC.Methods：A Varian CP7502 capillary column（25 m × 0.25 mm,0.25 μm） was used.The carrier gas was nitrogen with the flow rate of 30 ml·min^-1,the gas was hydrogen with the flow rate of 40 ml·min^-1and the oxidant gas was air with the flow rate of 400 ml·min^-1.The detector was an FID and the inlet temperature was 210℃.The temperature program was as follows：the initial column temperature was 40℃,and then risen to 200℃ with a rate of 15 ℃ ·min^-1.The split ratio was 100 ∶1 and the injection volume was 1μl.Results：Ethyl pyruvate had a good linear relationship within the range of 0.025 6-8.192 8 mg·ml^-1,and r was 0.999 8.The average recovery was 99.99% and RSD was 1.38%（n = 9）.Conclusion：The method is accurate,simple and rapid with good stability and high sensitivity,which can be used to determine the content of ethyl pyruvate.

关 键 词：丙酮酸乙酯 气相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]