应用多重PCR法对广东地区HBV进行基因型(A-F)分型  

Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China

作　　者：杨洁[1] 戴琳[1] 郭亚兵[1] 杨守昌[1] 王燕军[1] 骆抗先[1] 

机构地区：[1]第一军医大学南方医院感染内科,广东广州510515

出　　处：《第一军医大学学报》2002年第8期707-708,共2页Journal of First Military Medical University

基　　金：国家自然科学基金(39800125);广东省自然科学基金(980223)

摘　　要：目的建立乙型肝炎病毒(HBV)基因型(A-F)多重PCR分型方法,并利用该方法对广东地区HBV PCR阳性血清进行分型。方法将GenBank中114例HBV 全序列进行比较分析,找出每种基因型相对于其他五种基因型的独特序列,并根据这些独特序列设计出六对分别针对A-F基因型的特异引物。利用这六对引物建立HBV的多重PCR分型法。结果多重PCR与以前用PCR-限制温度长度多态型分析法的分型结果一致。对广州周边地区HBV携带者的初步分型结果显示,主要为B型和C型,分别占45.00%和38.75%,另外还有16.25%的D型。结论用多重PCR分型法准确易行,灵敏性高,便于推广应用。Objective To establish a convenient method for the genotyping of hepatitis B virus (HBV) using multiplex PCR. Method Based on the alignment of 114 complete nucleotide sequences of HBV DNA belonging to different genotypes, acquired from the GenBank, genotype-specific sequences were identified according to which 6 pairs of primers were designed corresponding to each genotype. Subsequent genotyping of HBV was performed using these primers that were added, either alone or in conjunction with others, into a multiplex PCR reaction tube, and HBV genotype was determined according to the length of amplified DNA. Result The genotyping result of multiplex PCR was consistent with that produced by PCR- restriction fragment length polymorphism as established by Lindh. We found in this study that among the HBV carriers in the vicinities Guangzhou of City, about 45% belonged to B genotype, 38.75% to C genotype and 16.75% to D genotype. Conclusion This multiplex PCR method is simple, convenient and more differential.

关 键 词：乙型肝炎病毒 基因型 多重聚合酶链反应 

分 类 号：R346[医药卫生—基础医学]