小檗碱对游离脂肪酸损伤的胰岛βTC3细胞的保护作用  

Protective Effect of Berberine on Lipoapoptosis in Pancreatic βTC3 Cells induced by Free Fatty Acid

作　　者：张娟[1,2] 刘文姝[1] 赵原[3] 张艺[1] 邹健[1] 李晓苗[1] 

机构地区：[1]第四军医大学西京医院内分泌代谢科,陕西西安710032 [2]三二0一医院内分泌科,陕西汉中723000 [3]第四军医大学药剂学教研室,陕西西安710032

出　　处：《现代生物医学进展》2016年第13期2442-2447,共6页Progress in Modern Biomedicine

基　　金：陕西省卫计委2014课题(2014D67);国家自然科学基金项目(81573746)

摘　　要：目的:观察小檗碱对棕榈酸损伤的胰岛βTC3细胞是否具有保护作用,并筛查出小檗碱起保护作用的有效浓度及合适的作用时间。方法:以胰岛βTC3细胞为研究对象,用棕榈酸构建脂毒性模型,MTT法筛选小檗碱起保护作用的有效浓度及时间;流式细胞技术检测胰岛βTC3细胞凋亡情况。结果:10.001-1μmol/L小檗碱作用βTC3细胞24、48、72 h,对细胞增殖有不同程度促进作用(与对照组比较P<0.05);随着作用时间的延长,低浓度小檗碱对βTC3细胞的保护作用增加,而1μmol/L浓度保护作用下降,10μmol/L及以上浓度出现细胞毒性作用(与对照组相比P<0.01),并呈浓度依赖性。2棕榈酸(0.2-1.0 mmol/L)对βTC3细胞的损伤作用具有浓度及时间依赖性(与对照组比较P<0.05)。3棕榈酸处理βTC3细胞不同时间后,小檗碱治疗组较模型组的细胞凋亡率显著降低(P<0.01)。结论:小檗碱对脂毒性损伤的胰岛βTC3细胞具有保护作用,且脂毒性作用时间越短,小檗碱的保护作用越好,随着脂毒性作用时间的延长,小檗碱的保护作用明显减弱。因此,建议临床上在脂代谢紊乱早期给予小檗碱干预以减轻甚至逆转游离脂肪酸导致的胰岛β细胞凋亡。Objective： To evaluate the protective effect of berberine on pancreatic 13TC3 cells lipoapoptosis induced by free fatty acids （FFA）, and the effective concentrations and treatment times of berberine were also investigated simultaneously. Methods： The re- search objects used were the pancreatic βTC3 cells, and palmitate was used to produce lipotoxieity model. The inhibitory actions of FFA on the proliferation of islet βTC3 cells were observed by using MTT assay, and the effective concentration of berberine and treatment time were investigated in details.Cell apoptosis was assessed by flow cytometry. Results： The results obtained from MTT indicated that berberine in concentration of 0.001 to 1 μmol/L can promote the proliferation of pancreatic βTC3 cells as compared to the untreated con- trois （P〈0.05）. The cell survival rate decreased obviously in the groups treated with 10, 50, 100 and 200 μmol/L berberine, indicating a high concentration ofberberine has a distinct cytotoxic effect in pancreatic βTC3 cells in a dose-dependent manner （P〈0.01）. Cell via- bility decreased after exposing to the free fatty acids in the concentration of 0.2 to 1.0 mmol/L for 24 to 72 hours. Illustrating the lipotoxi- city was in a time and dose dependent manner. Flow cytometry detection results were shown below, apoptosis was significantly increased upon treatment with palmitate as compared to the untreated controls （P〈0.05）. Berberine in the concentration of 0.1 μmol/L can signifi- cantly reduce palmitate-mediated apoptosis in pancreatic βTC3 cells （P〈0.01）. Conclusions： Berberine has a protective effect on lipotox- icity injury in pancreatic βTC3 Cells induced by Free Fatty Acid. Besides, lipoapoptosis the shorter the time, berberine protective effeet is better. With the extension of injury time, the protective effect diminished.

关 键 词：小檗碱 棕榈酸 胰岛βTC3细胞 脂毒性 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]