采用Ⅶ型胶原酶制作大鼠脑出血模型的技巧  

The Skills of Intracerebral Hemorrhage Rat Model by Type Ⅶ Collagenase Injection

作　　者：李东方[1] 赵海滨[1] 姜天元[1] 翟瑶瑶 杨丹丹[1] 

机构地区：[1]北京中医药大学第三附属医院

出　　处：《神经药理学报》2015年第4期22-25,共4页Acta Neuropharmacologica

基　　金：国家自然科学基金项目(No.81373590)

摘　　要：目的:研究Ⅶ型胶原酶脑内注入法构建大鼠脑出血模型的制作技巧。方法:运用立体定向技术及该文改进了的方法,向大鼠尾状核内缓慢注入Ⅶ型胶原酶,制作大鼠脑出血模型,通过观察其行为学变化,初步评价该模型建立的可行性和稳定性。结果:对150只SD大鼠进行脑出血造模,2只死亡,9例不成功,成功率为92.7%。结论:Ⅶ型胶原酶注射可建立理想稳定的脑出血模型,且操作简单,有较大推广价值。Objective:To study the skills of intracerebral hemorrhage model by injecting typeⅦ collagenase into brain region in rats.Methods:The animal model of intracerebral hemorrhage was induced by the injection of typeⅦ collagenase into caudate nucleus of rats with stereotactic instrument and improved methods. And then evaluate the feasibility and stability of the model through behavioral assessment initially.Results:150 rat models were made,two rats died,nine rats were not successful. The success rate of intracerebral hemorrhage model was 92.7%.Conclusion:The method of injecting typeⅦ collagenase can be used to build the animal model of intracerebral hemorrhage ideally and steadily. Because of the simple experiment operation,it has a great promotional vaule.

关 键 词：胶原酶 大鼠 脑出血 模型 

分 类 号：R965.1[医药卫生—药理学]