蛇床子素脂质体的制备及其质量评价  

Preparation and Quality Evaluation of Osthole Liposome

作　　者：张成龙[1] 张智峰[2] 孟宪勇[3] 董晓华[1] 

机构地区：[1]河北北方学院 [2]中国人民解放军93508部队医院 [3]河北北方学院附属第一医院

出　　处：《神经药理学报》2015年第4期26-32,共7页Acta Neuropharmacologica

基　　金：河北省科技支撑项目(No.13272704D);河北省教育厅重点项目(No.ZD2014065);张家口市科技局资助项目(No.0921098D);河北北方学院创新人才项目(No.CXRC1320)

摘　　要：目的:对蛇床子素脂质体最佳制备工艺及处方进行研究,并评价其质量。方法:采用薄膜-超声分散法制备蛇床子素脂质体,以包封率为评价指标,采用单因素和正交实验优化蛇床子素脂质体的制备工艺和处方,并测定其粒径和Zeta电位。结果:蛇床子素脂质体优化后的制备工艺和处方为:胆固醇与大豆卵磷脂质量比为1∶3,药脂比为3∶20,p H为7.9,成膜温度为45℃。按此处方工艺制备蛇床子素脂质体包封率在80%以上。平均粒径为(466.6±6.4)nm,平均Zeta电位为(-29.0±1.8)m V。结论:优选的处方和工艺条件可行且稳定,制备的蛇床子素脂质体包封率高,稳定性好。[ABSTRACTS] Objective:To research the best preparation technology and prescription, and to evaluate its quality.Methods:Osthole liposome was prepared through film-ultrasonic dispersion method. Encapsulation efficiency was taken as index,the preparation technology and prescription were optimized through single factor and orthogonal test,and particle size and Zeta potential were measured.Results:The optimal formulations were cholesterol/lecithin=1∶3, drug/lecithin =3∶20,pH=7.9,and at 45℃ reactive temperature. The entrapment efficiency was over 80%. Average particle size was(466.6±6.4) nm and Zeta potential was(-29.0±1.8) mV. Conclusion:The preparation prescription and technologywere feasible and stable,and osthole liposome has high entrapment efficiency and good stability.

关 键 词：蛇床子素 脂质体 薄膜-超声分散法 正交实验 

分 类 号：R965.2[医药卫生—药理学]