HPLC同时测定四数獐牙菜中3种有效成分含量  

Simultaneously Determination of Three Effective Components in Swertia tetraptera Maxim.

作　　者：赵英[1,2,3] 刘小翠[1,2,3] 廖志明[1,2,3] 卢永昌[1,2,3] 

机构地区：[1]青海民族大学药学院,青海西宁810007 [2]青海民族大学青海省青藏高原植物资源化学研究重点实验室,青海西宁810007 [3]青海民族大学青海省药物分析重点实验室,青海西宁810007

出　　处：《广西师范大学学报（自然科学版）》2016年第1期118-121,共4页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(81160554);青海省青藏高原植物化学重点实验室发展专项(2014-Z-Y13)

摘　　要：采用高效液相色谱法同时测定四数獐牙菜中獐牙菜苦苷、龙胆苦苷、獐牙菜苷的质量分数。结果显示,3种化学成分均达到基线分离,獐牙菜苦苷、龙胆苦苷、獐牙菜苷的线性范围分别为0.15-21.05mg/L（R=0.999 8）、0.25-28.05mg/L（R=0.999 9）、0.10-20.00mg/L（R=0.999 8）,回收率分别为99.1%（RSD=1.94%）、101.5%（RSD=2.05%）、96.0%（RSD=1.94%）,表明该测定方法快速,结果准确、可靠,可用于四数獐牙菜中獐牙菜苦苷、龙胆苦苷、獐牙菜苷的质量分数测定。RP-HPLC was used to determine the quantity and quality of swertiamarin,gentiopicroside and sweroside in Swertia tetraptera Maxim.The results showed that three compounds were base-isolated.The linear ranges of swertiamarin,gentiopicroside and sweroside were 0.052-1.030μg（R=0.999 8）,0.060-1.200μg（R=0.999 9）,and 0.050-0.99μg（R=0.999 8）respectively,the average recoveries were 99.1%（RSD=1.94%）,101.5%（RSD=2.05%）,and 96.0%（RSD=1.94%）respectively.This method is simple,rapid and reliable with good reproducibility,and can be used as the determination method for swertiamarin,gentiopicroside and sweroside in Swertia tetraptera Maxim.

关 键 词：高效液相色谱 獐牙菜苦苷 龙胆苦苷 獐牙菜苷 四数獐牙菜 

分 类 号：O415[理学—理论物理]