饥饿再投喂对鳜肌FSRP-1、FSRP-3和肠道PepT1基因表达的影响  

Effect of Starvating and Refeeding on Relative Expression of FSRP-1,FSRP-3 and PepT1 Gene in Mandarin Fish(Siniperca chuatsi)

作　　者：汪建华[1,2] 李玉珑[2] 陈敦学[1] 朱鑫[2] 刘知行[2] 张建社[2] 褚武英[2] 宾石玉[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541006 [2]长沙大学生物与环境工程系,湖南长沙410003

出　　处：《广西师范大学学报（自然科学版）》2016年第1期144-149,共6页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31472256;31560718;31230076);湖南省教育厅开放基金资助项目(15K011)

摘　　要：为探讨饥饿再投喂对鳜肌FSRP-1、FSRP-3和肠道中PepT1基因表达的影响,本研究采用qRT-PCR技术,测定饥饿7d再饱食一餐后0、1、3、6、12、24、48、96h条件下鳜肌中FSRP-1、FSRP-3和肠道中PepT1基因表达水平的变化,研究结果表明:FSRP-1基因表达在投喂后12h内显著升高(P<0.05),12-48h维持在较高表达水平,48h后显著降低(P<0.05);FSRP-3基因表达在投喂后1h内显著降低(P<0.05),12h达到峰值且表达量是1h的5倍,48h后显著降低(P<0.05);PepT1基因表达在投喂后6h内显著降低(P<0.05),48h时表达量最高。FSRP-1、FSRP-3和PepT1是与鱼类生长相关的重要基因,受饥饿再投喂的影响较大。To investigate postprandial changes in transcript abundance following a single satiating meal in mandarin fish（Siniperca chuatsi）going on a fast for 1week.The expression of FSRP-1and FSRP-3gene in fast myotomal muscle,as well as the PepT1 gene in small intestine were determined using quantitative real-time PCR at the following postprandial time points：0,1,3,6,12,24,48 and 96h.The results indicated that：FSRP-1transcripts were sharply up-regulated within 12 hof refeeding（P〈0.05）,the transcripts were maintained in the high level from 12 hto 48h,then it was significant downregulation in response to feeding;The FSRP-3gene expression was increased significantly（P〈0.05）in1hpostprandially,and reached peak expression levels at 12 hpostprandially,and decreased significantly after 48hpostprandially（P〈0.05）.The expression level of FSRP-3at 12 h was 5times higher compared with which at 1 h;The PepT1 gene had decreased expression in 6 h after the meal administration significantly（P〈0.05）,and reached maximal expression levels at 48 hpostprandially.FSRP-1,FSRP-3and PepT1 were absolutely necessary,associated with growth,which were greatly influence by starvation and refeeding.

关 键 词：鳜 饥饿再投喂 卵泡抑素相关蛋白1和3 肠道小肽转运载体 基因表达 

分 类 号：S965.127[农业科学—水产养殖]