黄酮对运动大鼠脂肪代谢酶与ERRα表达的影响  

Effects of Glycyrrhiza Flavonoids on Lipid Metabolism Enzyme and Expression of ERRαmRNA and Protein of Exhaustive Rats

作　　者：黄东[1] 李跃林[1] 卜凯[1] 黄文[1] 莫伟彬[1,2] 

机构地区：[1]广西师范大学体育学院,广西桂林541006 [2]药用资源化学与药物分子工程教育部重点实验室,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2016年第1期168-173,共6页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31460232;21562006);广西高校科研项目(YB2014384);"药用资源化学与药物分子工程教育部重点实验室"开放课题(CMEMR2012-B04);广西研究生创新项目(YCSW2015084)

摘　　要：为探讨耐力训练对大鼠脂肪代谢酶、雌激素受体关联受体α(ERRα)mRNA及蛋白表达与补充甘草黄酮的干预作用,选用SD雄性大鼠50只,每组10只,随机分为安静对照组(NC)、运动对照组(NE)、运动+补充甘草黄酮低(GFML,4g·kg^(-1)·d^(-1))、中(GFME,8g·kg^(-1)·d^(-1))、高(GFMH,12g·kg^(-1)·d^(-1))剂量组。对照组灌胃等量生理盐水,6周的力竭训练后,宰杀大鼠。根据试剂盒的方法测定大鼠血清肝脂酶(HL)、脂蛋白酯酶(LPL)、载脂蛋白CⅡ(ApoCⅡ)和载脂蛋白CⅢ(ApoCⅢ)含量和骨骼肌ERRαmRNA及蛋白表达水平,结果发现:运动对照组HL含量低于安静对照组(P<0.05),GFM各组HL含量高于运动对照组(P<0.05或P<0.01)。LPL含量在NE组高于NC组(P<0.05),GFM各组LPL含量高于安静对照组和运动组。ApoCⅡ含量在运动对照组高于安静对照组(P<0.05),GFM各组ApoCⅡ含量与安静对照组比较有显著性差异(P<0.05或P<0.01)。运动对照组ApoCⅢ含量低于安静对照组(P<0.05),GFME组和GFMH组ApoCⅢ含量与安静对照组比较有显著性差异(P<0.05)。NE组大鼠脂肪组织ERRαmRNA及蛋白表达水平高于NC组(P<0.05),在GFML、GFME和GFMH各组大鼠脂肪组织ERRαmRNA及蛋白表达都高于NC和NE组(P<0.05或P<0.01)。综上可见,补充甘草黄酮能够改善血脂代谢和调控酶水平在机体内的相对稳定性,从而防止脂代谢紊乱。In order to explore the intervention effect of endurance training of fat Rats on the lipid metabolism enzyme,Expression of ERRa mRNA and Protein and the complementary licorice flavonoids.Fifty healty male Sprague-Dawley rats were Randomly chosen for groups,each consisting of 10rats：the quiet control group（NC）,the exercise group（NE）,the administration of licoflavone in low dose exercising group（GFML,4g·kg-（-1）·d-（-1））,the administration of licoflavone in middle dose exercising group（GFME,8g·kg-（-1）·d-（-1））and the administration of licoflavone in high dose exercising group（GFMH,12g·kg-（-1）·d-（-1））.After the rats in control group were administrated of pure physiological saline at the same dose,the rats were killed after 6-week training.According to the kit method of determination of on lipid metabolism enzyme（HL,LPL,ApoCⅡand ApoCⅢ）in serum of rats and Expression of ERRa mRNA and Protein and The HL content in the quiet control group is lower than that of the exercise group（（P〈0.05）,The content of HL in GFM was higher than that of in the exercise group（（P〈0.05）.The content of LPL was higher than that of the quiet control group（P〈0.05）,The content of LPL in GFM was higher than that of the quiet control group and the exercise group.The content of ApoCⅡin the exercise group was higher than that of the quiet control（P〈0.05）,The content of ApoCⅡin GFM,compared with the quiet control group is significantly different（P〈0.05 or P〈0.01）,The content of ApoCⅢ is lower than that of the quiet control group（P〈0.05）,which means there are significantly different between GFME and GFMH and the quiet control group（P〈0.05）.The Adipose tissue Expression of ERR mRNA and Protein in NE was higher than that of the NC（P〈0.05）.The Adipose tissue Expression of ERR mRNA and Protein was higher than that of the NC and NE（P〈0.05 or P〈0.01）.Supplement of glycyrrhiza flavonoids can improve blood lipid metabolism and regulate

关 键 词：甘草黄酮 力竭运动 脂肪代谢酶 雌激素受体关联受体α 

分 类 号：R285.5[医药卫生—中药学]