基于纳米金催化的血清尿酸纸芯片的构建及应用  

Fabrication of Paper-based Microfluidic Chips and the Application on the Determination of Uric Acid in Serum Based on Gold Nanoparticle-assisted Catalysis

作　　者：赵甜甜[1] 陈雨晴[1] 张敏 王月荣[1] 章弘扬[1] 胡坪[1] 

机构地区：[1]华东理工大学化学与分子工程学院,上海市功能性材料化学重点实验室,上海200237 [2]华东理工大学药学院,上海市新药设计重点实验室,上海200237

出　　处：《高等学校化学学报》2016年第5期829-834,共6页Chemical Journal of Chinese Universities

基　　金：国家“重大新药创制”科技重大专项课题(批准号:2013ZX09507005);国家自然科学基金(批准号:81573397);中央高校基本科研业务费探索项目资助

摘　　要：建立了一种以纸芯片为平台,利用纳米金（Au NPs）的过氧化物模拟酶特性对血清中尿酸（UA）含量进行快速检测的方法.在改装的中性笔中灌注疏水性材料溶液,直接在滤纸上绘制所需要的图案,经干燥后形成纸芯片.将纳米金、四甲基联苯胺（TMB）和H_2O_2的混合液依次滴加于纸芯片检测区域,无色的TMB被氧化成蓝色,然后将待测样品滴加于蓝色区域,氧化态TMB被还原为无色,根据手机相机记录的检测区域灰度值计算试样中尿酸的浓度.实验优化了纳米金在纸芯片上的用量、反应时间和反应温度等参数,在最优实验条件下,检测尿酸的线性范围为10.6～125 mg/L,检出限为4.64 mg/L,加样回收率为94.8%～108.5%.该方法选择性良好,可用于测定血清样品中尿酸的含量.A simple and fast method for the determiantion of uric acid（ UA） in serum was developed using paper-based microfluidic chips and gold nanoparticles（ Au NPs） as peroxidase mimic. The paper-based microfluidic chips were fabricated by directly drawing on filter paper with a modified gel pen filled with hydrophobic solution and then dried. When the Au NPs and the mixed solution of TMB and H_2O_2 were droped on the detection zone of paper-based microfluidic chips,the colorless TMB was oxidated to blue substance.Then the sample was added onto the blue zone,the blue-oxidated TMB was reducted to colorless. Finally the picture of detection zone could be captured by a mobile phone camera and the concentration of UA could be caclulated according to the gray value of the detection zone. Parameters influencing the UA determination were investigated,including volume of Au NPs,reaction time and temperature. Under the optimized conditions,the detection limit of UA could be reached to 4. 64 mg/L,the linear range was from 10. 6 mg/L to 125 mg/L and the recovery was from 94. 8% to 108. 5%. The method could be applied to determining uric acid in serum.

关 键 词：纸芯片 纳米金 尿酸 

分 类 号：O657[理学—分析化学]