IBDV感染SPF雏鸡法氏囊中TLR3表达的动态变化  

Dynamic changes in the expression of TLR3 in the bursa of Fabricius of chicks infected with IBDV

作　　者：赵霞[1] 张瑞莉[1] 王海彬[1] 姜岩[1] 栾亚男 葛铭 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《黑龙江畜牧兽医》2016年第5期168-170,286,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31272533);黑龙江省自然科学基金项目(C201119)

摘　　要：为了研究鸡Toll样受体3(ch TLR3)基因在传染性法氏囊病病毒(IBDV)感染的雏鸡法氏囊内的表达情况,试验用IBDV感染14日龄SPF雏鸡,采用荧光定量PCR技术及间接免疫荧光技术检测法氏囊中TLR3基因及蛋白的表达情况。结果表明:感染组雏鸡法氏囊中TLR3 mRNA及蛋白表达均呈先升高后降低的趋势。说明TLR3参与雏鸡抵抗IBDV入侵的过程。To study the expression of chicken toll - like receptor 3 （ chTLR3 ） gene in the bursa of Fabricius of chicks infected with infectious bursal disease virus （IBDV） , 14 -dayold specific pathology free （SPF） chickens were treated with IBDV. The real -time fluorescence quantitative PCR and indirect immunofluorescence assay were used to detect the expressions of TLR3 gene and protein in the bursa of Fabricius. The result showed that the expressions of TLR3 mRNA and protein in the bursa of Fabricius of chicks in the infection group presented an increasing trend followed by a decreasing trend, The result indicates that TLR3 involves in the process that chicks resist the invasion of IBDV.

关 键 词：传染性法氏囊病病毒(IBDV) 鸡Toll样受体3 SPF雏鸡 法氏囊 基因 蛋白 

分 类 号：S852.659.5[农业科学—基础兽医学]