枳壳醇提物及其单体对小鼠原代神经细胞增殖作用的影响  

Effects of ethanol extract of Fructus aurantii and its monomers on the proliferation of mouse primary nerve cells

作　　者：罗小泉[1] 伍庆华[1] 郭权[1] 罗涛[1] 李中炼[1] 杨武亮[1] 

机构地区：[1]江西中医药大学,南昌330006

出　　处：《黑龙江畜牧兽医》2016年第5期194-196,287,288,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(81260612;81460575);江西省教育厅科技项目(GJJ14165)

摘　　要：为了探讨枳壳醇提有效部位及其所含部分单体成分对小鼠原代神经细胞增殖作用的影响,试验采用四甲基偶氮唑盐(MTT)测定细胞活力的方法,比较了枳壳醇提有效部位及其所含的川陈皮素、红橘素、伞形酮和葡萄内酯对小鼠原代神经细胞增殖作用的影响。结果表明:红橘素和伞形酮在试验浓度下对小鼠原代细胞增殖作用无显著影响;枳壳醇提有效部位(浸膏)、川陈皮素和葡萄内酯在试验浓度下对小鼠原代细胞生长有显著影响。说明枳壳醇提有效部位、葡萄内酯和川陈皮素对神经细胞生长有保护作用。To investigate the effects of the effective part of the ethanol extract of Fructus aurantii and its partial monomer contents on the proliferation of mouse primary neural ceils, four methyl thiazolyl tetrazolium （MTT） assay was used for the determination of cell viability in the test. The effects of the effective part of the ethanol extract of Fructus aurantii and its contained nobiletin, tangeritin, umbelliferone and gluconolac- tone on the proliferation of mouse primary neural cells were compared. The results showed that tangeritin and umbelliferone at the experimental concentrations had no significant effect on the proliferation of mouse primary ceils ; the effective parts of the ethanol extract of Fructus aurantii （extractum）, nobiletin and gluconolactone at the experimental concentrations had significant effects on the growth of mouse primary ceils. The results indicate that the effective part of Fructus aurantii, gluconolactone and nobiletin have protective effects on the growth of nerve ceils.

关 键 词：枳壳醇提物 川陈皮素 红橘素 葡萄内酯 伞形酮 原代神经细胞 增殖作用 

分 类 号：S853.75[农业科学—临床兽医学] Q813.11[农业科学—兽医学]