槟榔江水牛、摩拉水牛和荷斯坦奶牛血清中酶活性的测定  

Determination of serum enzyme activity in Binlangjiang buffaloes,Murrah buffaloes and Holstein cows

作　　者：彭洁[1] 贺珠婷 李卫真[1] 张永云[3] 苗永旺[1] 霍金龙[1] 刘超英[1] 俞大阔 赵敏 王绍卿[1] 尹康玲 

机构地区：[1]云南农业大学动物科学技术学院,昆明650201 [2]湖南省攸县畜牧水产局,湖南攸县412300 [3]云南农业大学农科专业基础试验教学示范中心,昆明650201 [4]文山州动物卫生监督所,云南文山663000 [5]云南省腾冲市畜牧兽医局,云南腾冲679100

出　　处：《黑龙江畜牧兽医》2016年第5期212-214,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31460649;31460582);云南省应用基础研究重点项目(2014FA032)

摘　　要：为了积累槟榔江水牛血清生化指标的基础数据,试验以10头槟榔江水牛为研究对象,对其血清中N-乙酰-β-D-氨基葡萄糖苷(NAGase)、γ-谷氨酰转移酶(γ-GT)、二酰基甘油酰基转移酶1(DGAT1)活性进行测定,并与摩拉水牛及荷斯坦奶牛进行比较。结果表明:槟榔江水牛、摩拉水牛、荷斯坦奶牛血清中NAGase活性差异不显著(P>0.05);槟榔江水牛、摩拉水牛血清中γ-GT活性显著高于荷斯坦奶牛(P<0.05),其中槟榔江水牛高于摩拉水牛,且差异不显著(P>0.05);槟榔江水牛、摩拉水牛血清中DGAT1活性极显著高于荷斯坦奶牛(P<0.01),其中摩拉水牛高于槟榔江水牛,且差异不显著(P>0.05)。To accumulate the basic data of serum biochemical indexes in Binlangjiang buffaloes, ten Binlangjiang buffaloes were used as research object in the test. The activities of serum N - acetyl - β - D - glucosaminidase （NAGase） , γ- glutamyl transferase （ γ- GT） , diacylglycerol acyltransferase I （ DGAT1 ） in Binlangjiang buffaloes were determined, which were compared with those in Murrah buffaloes and Holstein cows. The results showed that the difference of serum NAGase activity was not significant （ P 〉 0.05 ） among the three kinds of experimental animals ; serum γ- GT activity in Binlangjiang and Murrah buffaloes were higher than （ P 〈 0.05 ） that in Holstein cows, thereinto, serum γ- GT activity in Biulangjiang buffaloes was higher than that in Murrah Buffaloes, but the difference was not significant （P 〉 0.05 ） ; serum DGAT1 activity in Binlangjiang and Murrah buffaloes were extremely significantly higher （P 〈 0.01 ） than that in Holstein cows, thereinto, the activity in Murrah Buffaloes was higher than that in Binlangjiaug buffaloes, but the difference was not significant （P 〉 0.05 ）.

关 键 词：槟榔江水牛 摩拉水牛 荷斯坦奶牛 血清 酶 

分 类 号：S823[农业科学—畜牧学] Q814[农业科学—畜牧兽医]