高丛越橘“奥尼尔”的组织培养  

Tissue Culture of Vaccinium corymbosum ‘O'Neal'

作　　者：李森[1] 高丽霞[2] 刘念[3] 张施君[3] 

机构地区：[1]仲恺农业工程学院科技处,广东广州510225 [2]仲恺农业工程学院健康农业研究所,广东广州510225 [3]仲恺农业工程学院园艺园林学院,广东广州510225

出　　处：《北方园艺》2016年第9期102-105,共4页Northern Horticulture

基　　金：广东省科技计划资助项目(2013B020304006);广东省科技计划资助项目(广东省落叶果树工程技术研究中心)(2015B090903082);广东省科技厅产学研合作资助项目(2013B090200065)

摘　　要：以高丛越橘"奥尼尔"的带芽茎段为外植体,采用组织培养技术,研究了不同配比的ZT、6-BA、NAA和IBA植物激素对高丛越橘"奥尼尔"外植体诱导的影响。结果表明:最佳的外植体消毒条件为0.1%HgCl_24min,萌芽率可达71.4%。腋芽诱导的初代培养宜用WPM+1.0mg/L ZT培养基,侧枝能够萌发,苗高4.7cm;适宜的增殖培养基为WPM+2.0mg/L ZT,增殖系数达到13.0;试管苗的生根培养基为1/2WPM+0.1mg/L IBA。当试管苗长至5cm出瓶移栽,成活率可达80%以上。Taking stems with buds of Vaccinium corymbosum ‘O＇Neal＇as explants,the effect of different combination of ZT,6-BA,NAA and IBA added into the media were tested.The results showed that the optimal disinfection treatment was soaking into 0.1% HgCl2 for 4 min with induction rate of 71.4%.The optimal medium for bud induction was WPM＋1.0 mg/L ZT on which some branches germinated and plantlet length reached to be 4.7cm.The suitable proliferation medium was WPM＋2.0 mg/L ZT and the proliferation coefficient achieved to be 13.0.The optimum medium for root induction was 1/2WPM＋0.1mg/L IBA.The plantlets reached to be 5cm in length and could be transplanted outside and the survival rate exceeded 80%.

关 键 词：高丛越橘“奥尼尔” 组织培养 增殖 

分 类 号：S666.203.6[农业科学—果树学]