“新郁”葡萄离体再生体系的建立  

Establishment of Regeneration System of‘Xinyu'Grapevine in vitro

作　　者：骆萌[1] 王杰[1] 李玉玲 宋士任[1] 赵丽萍[1] 许文平[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]新疆维吾尔自治区葡萄瓜果研究所,新疆鄯善838200

出　　处：《北方园艺》2016年第9期109-113,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(31160381);上海市现代农业技术体系建设资助项目(沪农科产字(2015)第7号)

摘　　要：以"新郁"葡萄组培苗为试材,采用离体再生方法,探究了IBA+6-BA及IBA+TDZ2种激素组合的8个浓度配比对"新郁"葡萄离体叶片、叶柄和茎段的愈伤组织诱导及再生的影响。此外,通过比较红光、白光、蓝光、暗培养转红光、暗培养转白光、暗培养转蓝光6种光照条件下愈伤组织的诱导效率及分化潜力,确定了最适宜"新郁"葡萄离体组织培养的光照条件。结果表明:"新郁"葡萄离体再生体系在以叶片为外植体时不定芽再生效率最高;在最适宜的外源激素组合IBA 0.2mg/mL+6-BA 2.0mg/mL诱导下,不定芽再生率可达34.9%。6种光照条件中,以暗培养3周后转红光培养不定芽再生效果最佳,再生率、平均再生芽数、愈伤组织超氧化物歧化酶(SOD)活性和干物质含量均高于其它光质。Taking ‘Xinyu＇grapevine as test material,using regeneration in vitro method,the effect of hormone combination IBA＋6-BA and IBA＋TDZ on callus induction and adventitious bud regeneration of leaves,petioles,and stem was studied.In addition,the inducing efficiency and differentiation potential of callus had been evaluated under six light conditions（red light,white light,blue light,red light after dark culture,white light after dark culture,blue light after dark culture）to find the most suitable light condition for the in vitro regeneration system of‘Xinyu＇grapevine.The results showed that leaves should be used as explants for the regeneration of‘Xinyu＇grapevine.The most suitable hormone combination was IBA 0.2mg/mL＋6-BA 2.0mg/mL,while the most suitable light quality was red light（with3-week dark culture）.

关 键 词：“新郁”葡萄 再生 外植体 激素 光质 

分 类 号：S663.103.6[农业科学—果树学]