根结线虫生防细菌的筛选及鉴定  

Screening and Identification of Biocontrol Bacterial Against Root-knot Nematode

作　　者：王进福[1,2] 文才艺[1] 刘伟成[2] 董丹[2] 裘季燕[2] 刘霆[2] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002 [2]北京市农林科学院植物保护研究所,北京100097

出　　处：《北方园艺》2016年第9期121-124,共4页Northern Horticulture

基　　金：农业部公益性行业专项资助项目(201103018);北京市农林科学院青年科研基金资助项目(QNJJ201519)

摘　　要：以抗病番茄根际土壤、根为分离对象,采用稀释分离方法,进行了根结线虫生防细菌的筛选及鉴定。结果表明:从番茄根际土和根内分离出47株细菌,其中,根内细菌7株,根际细菌40株。经杀根结线虫活性检测,获得2株活性强且稳定的细菌菌株W49、W21,对根结线虫的校正死亡率均超过90.0%,对卵的孵化抑制率达96.0%。通过菌落形态特征、生理生化特征观察和16SrDNA序列分析,W49和W21分别被鉴定为简单芽孢杆菌(Bacillus simplex)和内生芽孢杆菌(Bacillus endophyticus)。Taking rhizosphere soil and roots of disease-resistant tomatoes as isolation objects,using dilution method,biocontrol bacterial against root-knot nematode were screened and identified.The results showed that 47 isolates were got among which 40 were from rhizosphere soil and 7were from roots.Strains W49 and W21showed nematicidal activity and egg hatching inhibition rate against Meloidogyne incogonita were over 90.0% and 96.0%,which were identified as Bacillus simplexand Bacillus endophyticus based on cultural and morphological characteristic and 16 SrDNA sequencing analysis.

关 键 词：根结线虫 生防细菌 活性检测 分类鉴定 

分 类 号：S482.292[农业科学—农药学]