高良姜素对LPS诱导THP-1细胞炎症的保护作用  

Protective effect of galangin on the LPS-induced inflammation in THP- 1 cells

作　　者：孔田玉[1] 张振辉[1] 胡欢[1] 辜洪娇 熊旭明[1] 

机构地区：[1]广州医科大学附属第二医院重症医学科,广东广州510260

出　　处：《中国急救医学》2016年第4期362-366,共5页Chinese Journal of Critical Care Medicine

基　　金：广东省科技厅项目（20128031800233）

摘　　要：目的探讨高良姜素对脂多糖（LPS）诱导人急性单核细胞白血病细胞株THP-1细胞炎症的保护作用及其可能的机制。方法MTS法检测细胞活性；酶联免疫吸附（ELISA）法检测TNF-α、MCP-1、IL-1β、IL-6的分泌；免疫印迹（Western blot）法检测NF—κB以及MAPK通路相关蛋白的变化。结果高良姜素在0～20μmol／L范围内对细胞活力无明显影响。LPS的用药浓度为500ng/mL；与LPS模型组相比，高良姜素2．5～20μmol／L能有效抑制炎症因子TNF-α、MCP-1、IL-1β、IL-6的释放（P〈0．05）；高良姜素20μmol／L能抑制NF—κB通路IKBα的磷酸化，从而减少核转录因子（NF）-κB的核转位，但对MAPK通路无影响。结论高良姜素对LPS刺激的THP-1细胞炎症具有一定的保护作用，其保护机制可能与其调控NF—κB通路，抑制IKBa的磷酸化，减少NF-κB的核转录，进而抑制TNF-α、MCP-1、IL-1β、IL-6的表达有关。Objective To observe the protective effect of galangin on the inflammation of human monocytic THP- 1 cells induced by lipopolysaccharide （LPS） and to explore it＇s underlying mechanisms. Methods Cell viability was detected by MTS method; ELISA was used to detect the expression of tumor necrosis factor- α （TNF-α）, monocyte chemotactic protein 1 （MCP- 1 ）, interleukin 1β （IL- 1β ） and interleukin 6 （IL-6）. The changes in protein levels of NF-κB and MAPK signal pathway were detected by Western blot. Results Galangin within 0-20 μmol/L had no effect on cell viability. Concentration of LPS was 500 ng/mL. Compared with the LPS model group, 2.5-20 μmol/L of galangin could inhibit the release of TNF-α, MCP-1, 1L-1β, IL-6 （P 〈 0.05）. 20 μmol/L of Galangin could inhibit the phosphorylation of IκBα, reduce the occurrence of NF-κB nuclear translocation. However, the protein levels of MAPK signal pathway were unaltered by galangin. Conclusion Galmagin has protective effects on THP-1 cells stimulated by LPS. The mechanism of galangin may be related to regulating the NF-κB signal pathway, inhibiting the phosphorylation of IκBα, reducing the occurrence of NF-κB nuclear translocation, thereby inhibiting the release ofTNF-α, MCP-1, IL-1β and IL-6.

关 键 词：高良姜素 THP-1 脂多糖 抗炎 核转录因子-ΚB MAPK 

分 类 号：R631.3[医药卫生—外科学]