头孢唑啉钠与木瓜酶相互作用初探  

Preliminary Study on the Interaction between Cefazoline Sodium and Papain

作　　者：席小莉[1] 刘芳[1] 杨曼曼[1] 陈廷贵[1] 刘斌[1] 

机构地区：[1]化学生物学与分子工程教育部国家重点实验室山西大学分子科学研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2016年第2期276-280,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(21271122);山西省自然科学基金(2013011010-2);山西省高等学校科技创新项目(STIP)(20111002);山西省回国留学人员科研资助项目(2015-021)

摘　　要：利用光谱、分子模拟研究头孢唑啉钠和木瓜酶之间的作用。给体-受体间距离为r=1.53nm。当温度为298K时,结合常数为1.79×104 L·mol-1;△S0=45.56J·mol-1·K-1。同时发现,荧光猝灭的主要原因是静态猝灭。分子模拟结果:作用力主要为氢键,范德华力及疏水作用。另外,头孢唑啉钠的氨基氢与甘氨酸氧之间氢键键长为1.91。The interaction between cefazoline sodium and papain was investigated by spectroscopy and molecular simulation.We calculated the binding distance between the offer and the receptor is r=1.53 nm.When the temperature is 298,the binding constant K was 1.79×104 L·mol-1.The statistic quenching was the main reason for the fluorescence quenching process.Molecular simulations show that：the binding types of cefazoline sodium and papain are hydrogen bond,van der Waals＇ forces and hydrophobic interaction.The bond length between hydrogen atom in amino of cefazoline sodium and oxygen of glycine is 1.91.

关 键 词：头孢唑啉钠 给体和受体 同步荧光 

分 类 号：R969[医药卫生—药理学]