Eaf2在胃癌中的表达及其对细胞增殖、迁移和侵袭的影响  

Effects of Eaf2 on Cell Proliferation,Migration and Invasion of MGC-803 Gastric Cancer Cells

作　　者：李峰[1] 杜军[1] 梁爱华[1] 

机构地区：[1]山西大学生物技术研究所化学生物学与分子工程教育部重点实验室,太原030006

出　　处：《山西大学学报（自然科学版）》2016年第2期307-312,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31372199)

摘　　要：为了揭示Eaf2对胃癌MGC-803细胞增殖、侵袭和迁移的影响,使用定量Real-time PCR分析了50例胃癌组织(C)及其癌旁(N)中Eaf2的表达。用pcDNA3.1载体构建过表达Eaf2质粒(pcDNA-Eaf2),以空载体pcDNA3.1(pcDNA-NC)作为对照,利用CCK-8法检测细胞增殖,Transwell小室和划痕实验检测细胞侵袭能力和迁移能力。Real-time PCR结果显示42例(84%)胃癌组织Eaf2表达下调,提示Eaf2作为抑癌基因起作用。过表达Eaf2能显著抑制MGC-803细胞的增殖、迁移和侵袭能力(P<0.05)。结果提示Eaf2表达下调可能与胃癌的发展进程有关。To investigate the effects of Eaf2 on cell proliferation,migration and invasion of gastric cancer cell lines MGC-803,the level of Eaf2 expression was detected by real-time RT-PCR in 50 paired gastric cancer tissues and their adjacent normal tissues.Eaf2 overexpression was achieved by transfection of construct pcDNA-Eaf2 into MGC-803 cells.The proliferation,migration and invasion of MGC-803 cells were detected by the CCK-8,scratch wound and transwell assay respectively.The real-time PCR results indicated that 42cases（84%）of gastric cancer tissues were decreased.Think that Eaf2 plays an important role in gastric cancer as a tumor suppressor,overexpression of Eaf2 can effectively inhibit the proliferation,invasion and migration of MGC-803cells（P〈0.05）.The results suggested that down-regulation of Eaf2 had a correlation with the progression of gastric cancer.

关 键 词：Eaf2 MGC-803细胞 胃癌 增殖 迁移 侵袭 

分 类 号：Q527[生物学—生物化学]