光合生物制氢系统菌种连续培养装置的设计及实验研究  

DESIGN AND OPERATION OF CONTINUOUS BACTERIA-CULTIVATING DEVICE FOR BIOLOGICAL HYDROGEN PRODUCTION SYSTEM

作　　者：魏斌[1] 周雪花[1] 王毅[1] 张志萍[1] 张全国[1,2] 

机构地区：[1]河南农业大学生物质能源河南省协同创新中心,郑州450002 [2]农业部可再生能源新材料与装备重点实验室,郑州450002

出　　处：《太阳能学报》2016年第4期985-990,共6页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(51376056);国家高技术研究发展(863)计划(2012AA051502);教育部博士点基金优先领域项目(20134105130001)

摘　　要：根据光合生物制氢系统连续运行的菌种要求以及光合产氢菌种HAU—M1的生长特性，设计一套针对大中型光合生物制氢系统的光合细菌菌种培养装置，研究该装置对菌种的连续培养和菌种稳定供应的影响因素，优化其初始接种量、水力停留时间等工艺参数并进行连续性培养及运行实验。研究表明：在初始接种量15％，水力停留时间48h，温度30℃，光照强度2080Lux，pH值6．8的条件下，菌种培养装置出口培养液浓度可稳定在10％～20％，出口流量维持在约28．96L／h，可满足光合生物制氢系统对菌种稳定供应的实际需要，验证了光合细菌菌种培养装置应用于光合生物制氢系统连续性运行的技术可行性。According to the requirements of the photosynthetic bacteria HAU-M 1 cultivation and the actual needs of systems operation, a set of bacteria-cultivating device for photo-fermentative bio-hydrogen production （PFBP）system was designed. Several factors affecting the continuous cultivation and steady supply of the cultivating device were investigated. Through the culture assays, the initial bacterial inoculum size, hydraulic retention time （HRT） , culture temperature, pH value and illumination intensity were optimized when adopting continuous cultivating system. Under the conditions that inoeulum size of 15%, HRT of 48 h, temperature of 30 ℃, illumination intensity of 2080 Lux and pH value of 6.8, the bacteria concentration at the discharge hole was maintained at 10%-20%, and the outlet flow was kept around 28.96 L/h. The bio-hydrogen production process was carried out using the optimized culture parameters. The results show that the continuous cultivating system can satisfy the practical requirement of bacteria supply for the PFBP process, and the technical feasibility of the bacteria-cultivating device is verified.

关 键 词：生物制氢系统 HAU-M1 菌种培养 装置设计 连续培养 

分 类 号：TK6[动力工程及工程热物理—生物能]