饮片规格对何首乌化学成分溶出和肝细胞毒性的影响  

Influence of specification on chemical composition of dissolution and hepatocytes toxicity of Polygonum multiflorum

作　　者：李雨萌[1,2] 李瑞煜[2] 牛明[2] 李春雨[2] 柏兆方[2] 冯五文 章从恩[2] 谭鹏[2] 黄之镨[1] 马伟光[1] 王伽伯[2] 肖小河[3] 

机构地区：[1]云南中医学院中药学院,云南昆明650500 [2]解放军302医院全军中医药研究所,北京100039 [3]解放军302医院中西医结合肝病诊疗与研究中心,北京100039

出　　处：《中国中药杂志》2016年第6期1033-1039,共7页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2015ZX09501-004-001-008);国家公益性行业科研专项(201507004-04);北京科技新星计划项目(xx2016098)

摘　　要：根据不同规格何首乌的煎煮成分对肝细胞毒性的差异,探讨何首乌饮片规格对其安全性的影响。该研究通过建立何首乌8种主要化学成分的含量测定方法,测定不同规格何首乌水煎液样品中各化学成分的含量,并以其对正常人L02肝细胞的抑制率作为肝毒性的评价指标,采用多元相关分析,试图找出与毒性相关的化合物。结果显示不同规格何首乌饮片成分溶出差异较大,何首乌打粉饮片的肝细胞毒性显著强于何首乌块状饮片;通过多元相关分析发现了3个与何首乌肝毒性密切相关的成分。结果揭示了饮片规格与何首乌肝细胞毒性的关系,其中粉状规格饮片毒性相对较大,有一定的肝损伤风险,应予以高度重视。According to different toxicities of various aqueous extracts of Polygonum multiflorum on hepatocyte,the impacts of chemical composition on the safety of P. multiforum was studied. In this study,8 main chemical compositions in aqueous extracts of P. multiflorum were determined by the established HPLC method; at the same time,the inhibition ratios of different aqueous extracts of P. multiflorum on L02 cell were determined. Afterwards,the potential compounds related to the toxicity of P. multiforum were tentatively found through a multiple correlation analysis. The results showed that P. multiforum with different chemical compositions exhibited great differences in dissolution. The hepatocyte toxicity of P. multiflorum powder was much greater than P. multiflorum lumps. In addition,three constituents closely related to toxicity of P. multiflorum were found by multiple correlation analysis. The study revealed that chemical composition of P. multiflorum is closely related to the hepatotoxicity,and the hepatotoxicity of P. multiflorum powder is greater than that of other dosage forms. This study indicates that P. multiflorum with different chemical compositions show varying toxicity,which therefore shall be given high attention.

关 键 词：何首乌 饮片规格 肝毒性 多元相关分析 顺式二苯乙烯苷 大黄素 

分 类 号：R284[医药卫生—中药学] R285.1[医药卫生—中医学]