牡丹和芍药种皮、种仁及种皮提取物中10种茋类成分含量及抗氧化测定  

Determination of ten stilbenes and their antioxidant activity of peony seed coat,seed kernel and seed coat extracts

作　　者：何春年[1,2] 毕武[1,2] 申洁[1,2] 彭勇[1,2] 肖培根[1,2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]国家教育部中草药物质基础与资源利用重点实验室,北京100193

出　　处：《中国中药杂志》2016年第6期1081-1086,共6页China Journal of Chinese Materia Medica

基　　金：国家"十二五"科技支撑计划项目(2012BAI28B002)

摘　　要：常用中药牡丹和芍药种子中含有茋类成分,具有多种药理活性,为了开发和利用牡丹种子资源,该文选择种子中含量相对较高的10种茋类成分suffruticosol A,suffruticosol B,suffruticosol C,trans-resveratrol,cis-ε-viniferin,trans-ε-viniferin,cissuffruticosol D,cis-gnetin H,trans-suffruticosol D和trans-gnetin H为指标进行含量测定。采用HPLC-DAD,YMC-pack ODS-A色谱柱(4.6 mm×250 mm,5μm);流动相以甲醇-水梯度洗脱,流速为1.0 m L·min-1;柱温25℃;检测波长为230 nm。同时采用清除DPPH自由基的方法初步评价其抗氧化作用。结果表明:所建立的方法能够同时测定10种茋类成分的含量;牡丹和芍药种子中的茋类成分主要集中于种皮部位(总质量分数达16.7%以上),种仁中含量很低(总质量分数不到0.3%),种皮提取物富含茋类成分,总含量达75%以上。种皮提取物及10种茋类成分多具有较好的抗氧化潜力。该文对牡丹和芍药资源的综合利用,特别是种皮资源的有效开发和利用奠定了基础。The seed of tree peony and herbaceous peony contained a variety of stilbenes which possess many pharmacological activities,such as antitumor,anti-inflammatory,allergy and neuraminidase inhibition. In order to develop and use peony seed resources,a simple and sensitive HPLC-DAD was developed for simultaneous determination of 10 stilbenes in peony samples,i. e.,suffruticosol A,suffruticosol B,suffruticosol C,trans-resveratrol,cis-ε-viniferin,trans-ε-viniferin,cis-suffruticosol D,cis-gnetin H,trans-suffruticosol D and trans-gnetin H. At the same time,the scavenging activity of DPPH free radicals was employed to evaluate their antioxidant effect.The results showed that the 10 stilbenes are mainly present in peony seed coat（ total content of more than 16. 7%） compared to peony seed kernel（ total content less than 0. 3%）,and can be enriched in the extract of peony seed coat（ total content of more than 75%）The extract of peony seed coat and 10 stilbenes exhibited significant antioxidant properties. This work provides a foundation for comprehensive utilization of the tree peony and herbaceous peony seed resources.

关 键 词：牡丹 芍药 种皮 茋类 白藜芦醇 

分 类 号：R284.1[医药卫生—中药学]