茵栀黄注射液抗胆汁淤积药效成分的筛选及其作用机制研究  

Screening of effective ingredients of Yinzhihuang injection against cholestasis and their mechanism

作　　者：谭桢[1] 刘爱明[2] 罗敏[2] 郭宾[1] 

机构地区：[1]湖南师范大学化学化工学院,湖南长沙410081 [2]宁波大学医学院,浙江宁波315211

出　　处：《中国中药杂志》2016年第6期1113-1118,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81274178;81273582;81302848)

摘　　要：筛选茵栀黄注射液中的主要药效成分,评价其调节胆汁酸代谢相关基因,发挥护肝作用的机制。选用雄性健康ICR小鼠30只,分为6组:正常对照组、模型对照组、绿原酸组、栀子苷组、黄芩苷组、滨蒿内酯组。检测肝功能、肝组织病理学变化以及胆汁酸代谢相关基因的表达。绿原酸组小鼠血清ALT,AST,ALP,TBA水平分别为(15.89±2.53),(18.32±2.56),(26.38±9.87)U·L^(-1),(40.63±7.67)μmol·L^(-1),栀子苷组小鼠分别为(20.54±2.36),(24.28±5.19),(35.09±5.03)U·L^(-1),(42.86±7.11)μmol·L^(-1),均低于模型对照组(59.52±10.94),(128.37±17.97),(169.52±9.62)U·L^(-1),(132.50±33.00)μmol·L^(-1)。肝组织病理学分析显示,绿原酸组、栀子苷组小鼠肝细胞的坏死、炎性细胞浸润程度与模型对照组比较均显著减轻。荧光定量Q-PCR检测显示,给予绿原酸、栀子苷治疗后,胆汁酸代谢相关基因mRNA的变化较模型对照组均发生了代偿性的逆转。茵栀黄注射液中的绿原酸和栀子苷能有效改善胆汁淤积、肝功能及肝脏病理性损伤,并逆转ANIT引起的胆汁酸代谢相关基因mRNA变化,从而起到肝保护作用。Chinese herbal medicinal formulation Yinzhihuang injection is widely used in clinic for jaundice and chronic liver diseases in eastern Asian countries. However,the pharmacologically active components and the underlying mechanism are unclear. In this study,30 male ICR mice were randomly assigned into 6 groups： normal control,model control,chlorogenic acid group,geniposide group,baicalin group and wogonoside group. The liver function,liver pathological changes and bile acid metabolism-related gene expression in mice were assayed. The serum levels of ALT,AST,ALP,TBA in chlorogenic acid group（ 15. 89 ± 2. 53）,（ 18. 32 ±2. 56）,（ 26. 38 ± 9. 87） U· L- 1,（ 40. 63 ± 7. 67） μmol·L^（-1）,respectively and geniposide group（ 20. 54 ± 2. 36）,（ 24. 28 ±5. 19）,（ 35. 09 ± 5. 03） U · L- 1,（ 42. 86 ± 7. 11） μmol·L^（-1）,respectively were lower than those in the model control group（ 59. 52 ± 10. 94）,（ 128. 37 ± 17. 97）,（ 169. 52 ± 9. 62） U·L^（-1）,（ 132. 50 ± 33. 00） μmol·L^（-1）,respectively. Hematoxylin-eosin staining showed necrosis,infiltration of inflammation cell in chlorogenic acid group and geniposide group were milder than those in the model control group. Q-PCR analysis revealed the expression of bile acid metabolism-related genes was normalized after treatment with chlorogenic acid or geniposide. Chlorogenic acid and geniposide improved the liver injury and cholestasis effectively,and reversed the mRNA expression of bile acid metabolism-related genes induced by ANIT. So,chlorogenic acid and geniposide were protective for cholestasis,suggesting their pharmacodynamic effect in Yinzhihuang injection.

关 键 词：茵栀黄注射液 绿原酸 栀子苷 胆汁酸代谢 肝内胆汁淤积 

分 类 号：R285.5[医药卫生—中药学]