石墨炉原子吸收光谱法测定尿中铬的方法研究  

Determination of urinary chromium by graphite furnace atomic absorption spectrometry

作　　者：刘浏 胡晓宇 陈斌 傅佳 

机构地区：[1]北京市预防医学研究中心职业卫生所,北京100020

出　　处：《职业与健康》2016年第7期899-901,共3页Occupation and Health

摘　　要：目的建立采用氯化钯为基体改进剂测定人体尿液中铬的石墨炉原子吸收方法。方法尿样用1%稀硝酸稀释后,氯化钯作为基体改进剂进行石墨炉原子吸收法测定尿中铬的含量。研究方法按照《生物材料中化学物质测定方法》的要求进行。结果筛选出氯化钯作为测定人体尿液中铬的基体改进剂。该方法检测铬时在0~40μg/L范围内呈线性关系,浓度与吸光度的回归方程为Y=0.017 6X＋0.036 5;相关系数为0.999 7,最低检出限为0.353μg/L;不同浓度的相对标准偏差为0.28%~1.97%,加标回收率在97.9%~102.0%之间。样品在试管中分别于-20和4℃环境下保存,在1~14 d内均保存良好。结论采用氯化钯作为基体改进剂,用于石墨炉原子吸收光谱法测定尿液中的铬元素,该方法具有灵敏度高、稳定性好、操作简便、检出限低等特点,适用于尿中铬含量的测定。[Objective]Using palladium chloride as matrix modifier,to establish a method for determination of urinary chromium by graphite furnace atomic absorption spectrometry.[Methods]Using palladium chloride as matrix modifier,the urinary samples were diluted with 1% nitric acid,and the concentration of chromium in urine was determined by graphite furnace atomic absorption spectrometry.The laboratory examination was conducted according to the Determination methods of chemicals in biological materials.[Results]The palladium chloride was used as the matrix modifier in determination of urinary chromium.The standard curve was linear in the content ranges of 0-40 μg/L,and the regression equation was Y =0.017 6X ＋0.036 5,r =0.999 7.The detection limit was 0.353 μg/L.The relative standard deviation（RSD）was 0.28%-1.97%,and the recovery rate was 97.9%-102.0%.The samples could be stably stored for 14 days in-20 ℃ and 4 ℃.[Conclusion]The palladium chloride can be used as the matrix modifier in determination of urinary chromium by graphite furnace atomic absorption spectrometry,and the method is simple and rapid,with high sensitivity and low detection limit,which is suitable for determination of chromium in urine.

关 键 词：石墨炉原吸收 尿铬 基体改进剂 

分 类 号：R115[医药卫生—公共卫生与预防医学]