肠S9温孵法研究川芎多成分肠吸收过程中的肠壁代谢  

Evaluation for impacts of gut wall metabolism of multiple components in Chuanxiong Rhizoma during intestinal absorption with S9 incubation

作　　者：杨文宁[1] 李博[1] 王豪[1] 陈新晶[1] 杨立媛 刘洋[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中国中药杂志》2016年第7期1188-1191,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81274042)

摘　　要：生物药剂学分类系统(biopharmaceutics classification system,BCS)研究中,肠吸收性评价实验要考虑吸收过程中的肠壁代谢问题,因此该研究选用大鼠肠S9对川芎中多成分的肠壁代谢进行研究,为进一步BCS肠吸收性评价实验厘清代谢影响。利用大鼠肠S9对川芎水提液进行体外温孵实验研究,通过HPLC指纹图谱技术,对比研究多成分色谱峰面积温孵前后的变化。结果表明川芎水提液经肠S9温孵后,以指纹图谱表征的多成分谱图中,洋川芎内酯I等多个成分发生肠壁代谢,有1个代谢转化成分生成。阿魏酸等多个成分不发生肠壁代谢。因此,建议下一步川芎的BCS肠吸收性评价实验可以对不发生肠壁代谢成分直接测定,而对肠壁代谢成分的肠吸收性评价则需要探索更合适的实验方法。The information of drug deposition in the intestine is required in the study for the drug absorption in biopharmaceutics classification system（ BCS）. To illustrate the impacts of gut wall metabolism on the absorption,metabolism of multiple components in Chuanxiong Rhizoma in gut wall was tested by rat S9 incubation in vitro. The chemical fingerprint technology was used in this study to simultaneously detect multiple components in Chuanxiong,and peak areas before and after S9 incubation were compared. The results showed that senkyunolide I and several constituents were metabolized by gut wall,and one new metabolite was founded. However,ferulic acid and other compounds remained unchanged after incubation. Therefore,the subsequent intestinal permeability of multiple components in Chuanxiong that were not metabolized in the intestine was suggested to be detected directly by in situ single-pass intestinal perfusion（ SPIP）. Nonetheless,the intestinal permeability of the constituents that were metabolized in the intestine shall be explored by appropriate approaches.

关 键 词：生物药剂学分类系统 肠S9 多成分 

分 类 号：R285[医药卫生—中药学]