盐酸小檗碱在黄连水煎液中的生物药剂学分类系统属性研究  

Study on property of biopharmaceutics classification system of berberine in Huanglian decoction

作　　者：隗丽[1] 朱美玲[1] 董月柳 董玲[1] 刘洋[1] 陈江鹏[1] 尹秀文[1] 

机构地区：[1]北京中医药大学

出　　处：《中国中药杂志》2016年第7期1192-1197,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473362);北京中医药大学在读研究生项目(2015-JYB-XS092)

摘　　要：中药生物药剂学分类系统的基础研究之一是在多成分环境下对单一成分进行研究,即除成分自身溶解性和渗透性外,需结合多成分环境下造成的溶解性和渗透性各自提升度进行分类研究。该研究以小檗碱为主要研究对象,探究其在黄连水煎液中的水溶解性及肠渗透性变化规律。其中采用经典摇瓶法和高效液相色谱法测定小檗碱在不同p H缓冲液及不同浓度的黄连水煎液中的溶解度,并分别采用在体单向灌流模型及肠道灌流并行采血模型进行小檗碱的肠吸收和入血吸收的研究。The study of single component in the multicomponent environment is one of the basic researches for biopharmaceutics classification system of Chinese materia medica（ CMMBCS）. That is to say,the classification research shall be based on the respective lift of solubility and permeability in the multicomponent environment,besides solubility and intestinal permeability of the single component. We chose berberine as the main research object to investigate the changes of its solubility and intestinal permeability in Huanglian decoction. Shake-flask and HPLC were used to detect the solubility of berberine in different p H buffer solutions and different concentrations of Huanglian decoction. In situ single-pass intestinal perfusion（ SPIP） and intestinal perfusion with venous sampling（ IPVS）were carried out to study berberine＇s intestinal absorption and absorption into blood,respectively.

关 键 词：黄连 小檗碱 溶解性 肠渗透性 中药生物药剂学分类系统 

分 类 号：R285.5[医药卫生—中药学]