小肠黏膜下层冷冻凝胶用于细胞移植支架的研究  

Small intestinal submucosa cryogel as scaffold for cell transplantation

作　　者：廖建贵[1] 李喆[1] 曹川[1] 葛良鹏 李世荣[1] 

机构地区：[1]第三军医大学西南医院整形美容外科,重庆400038 [2]重庆市畜牧科学研究院,重庆400039

出　　处：《第三军医大学学报》2016年第10期1060-1065,共6页Journal of Third Military Medical University

基　　金：国家高技术研究发展计划(863计划;2014AA020705);国家自然科学基金青年科学基金(31500790)~~

摘　　要：目的利用猪小肠黏膜下层(small intestinal submucosa,SIS)制备一种可用于细胞移植的具有形态记忆功能的新型支架材料。方法将脱细胞SIS粉末溶解,通过自制混匀装置,用不同浓度的碳化二亚胺冷冻交联制备不同参数的SIS冷冻凝胶。检测弹性形变及形态记忆功能。扫描电镜观察并测定孔径大小,检测吸水率、体外降解率和机械性能,观察小鼠成纤维细胞在支架上的黏附生长情况。结果 SIS冷冻凝胶压缩达85%以上仍可复原,可通过注射器注射。扫描电镜提示具有疏松多孔结构,孔隙相通,孔径大小为(106.63±28.90)μm。随着碳化二亚胺浓度的升高,吸水率、细胞毒性差异无统计学意义,降解速率逐渐减缓,机械强度逐步提升。小鼠成纤维细胞在SIS冷冻凝胶上呈线形、纺锤形生长,有大量伪足,部分融合成片紧贴于材料表面及孔隙内壁。结论具有弹性形变和形态记忆功能的可注射SIS冷冻凝胶生物、物理性能良好,是一种良好的细胞移植支架材料。Objective To prepare a new scaffold with shape memory properties for cell transplantation by using porcine small intestinal submucosa（ SIS）. Methods The acellular SIS powder was dissolved, mixed with different concentrations of 1-ethyl-3-（ 3-dimethyllaminopropyldvb） carbodiimide hydrochloride（ EDC） with a self-made mixing device,and crosslinked in the frozen condition. The elastic deformation and shape memory capability were detected. Scanning electron microscopy was used to perform morphological observation and detect the pore size,and the water absorption ability,biodegradation behavior and mechanical properties were detected. The mice fibroblasts were used for cell adhesion and proliferation study. Results SIS cryogel could be compressed over 85% and injected through a needle without any damage. The cryogel had porous and interconnected pore structure with average pore size of 106. 63 ±28. 90 μm. There was no significant difference in water absorption ability and cytotoxicity at different concentrations of EDC. But the degradation became slow and mechanical strength was enhanced when the EDC concentration increased. Cells grew linearly and spindly on the SIS cryogel,and stretched out plenty of pseudopodiums. Some sections were integrated into film and stuck to the surface and pore walls. Conclusion Injectable SIS cryogel with elastic deformation and shape memory capability can be used as cell transplantation scaffold because of good biocompatibility and physical properties.

关 键 词：小肠黏膜下层 支架 冷冻凝胶 

分 类 号：R318.08[医药卫生—生物医学工程] R322.45[医药卫生—基础医学]