尿源干细胞促进脊髓损伤后神经源性膀胱恢复  

Effect of urine-derived stem cell transplantation on recovery of neurogenic bladder in rats after spinal cord injury

作　　者：张腾[1] 董兴有 刘骞[1] 吴超[1] 李佳[1] 夏六兵 胡晓燕[1] 宋波[2] 邓建平[1] 李龙坤[1] 

机构地区：[1]第三军医大学新桥医院泌尿外科,重庆400037 [2]第三军医大学西南医院全军泌尿外科研究所,重庆400038

出　　处：《第三军医大学学报》2016年第10期1073-1077,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81170704);国家自然科学基金青年科学基金(81300630)~~

摘　　要：目的观察大鼠神经源性膀胱模型功能和组织学变化,探索尿源干细胞对大鼠神经源性膀胱的修复治疗作用。方法 45只健康清洁的正常SD大鼠按照完全随机法分为正常组、损伤组、干细胞组,每组15只。治疗组建模7 d后,尾静脉注射尿源性干细胞。28 d后,尿动力学检测大鼠尿动力各项指标,肌条实验观察膀胱收缩情况,Western blot观察P2Y4在膀胱中的表达,TUNEL染色观察细胞凋亡情况。结果干细胞组大鼠膀胱湿质量(0.31±0.01)g和膀胱湿质量/体质量值(1.19±0.41)、尿动力指标[收缩时间(112.75±16.52)s、离体膀胱逼尿肌肌条指标[收缩时间(16.47±2.29)s]、膀胱肌细胞凋亡率(28.22±11.08)%均优于损伤组[膀胱湿质量(0.66±0.07)g,膀胱湿质量/体质量值(2.30±0.26),尿动力指标:收缩时间(59.00±5.68)s,离体膀胱逼尿肌肌条指标:收缩时间(19.80±3.77)s,膀胱肌细胞凋亡率(59.80±14.18)%]。Western blot检测结果显示干细胞组的蛋白P2Y4的表达低于损伤组。结论尿源干细胞可促进脊髓损伤后大鼠神经源性膀胱的功能恢复。Objective To observe the function and histological changes of neurogenic bladder,and investigate the effect of urine-derived stem cell transplantation on the neurogenic bladder in rats after spinal cord injury（ SCI）. Methods Forty-five Sprague-Dawley（ SD） rats were randomly divided into normal group,SCI-induced group,and SCI-induced and urine-derived stem cell transplantation group（ n = 15 for each group）. On the 7th day after induction of SCI,the rats in the stem cell transplantation group were injected with urine-derived stem cells. After 28 d,urodynamic test,in vitro detrusor smooth muscle strips and bladder wet weight were used to measure the function of bladder. Western blotting was employed to detect the expression of P2Y4 in each group. TUNEL assay was performed to test the apoptosis ratio of bladder cells.Results The SCI-induced and urine-derived stem cell transplantation group was better in bladder wet weight（ 0. 31 ± 0. 01 g）,bladder wet weight/body mass（ 1. 19 ± 0. 41）,urodynamic parameter（ contraction intermittence 112. 75 ± 16. 52 s）,in vitro detrusor smooth muscle strips（ contraction intermittence 16. 47 ±2. 29 s）,and bladder cell apoptosis ratio [（ 28. 22 ± 11. 08） %]compared with the SCI-induced group [0. 66 ±0. 07 g,2. 30 ± 0. 26,59. 00 ± 5. 68 s,19. 80 ± 3. 77 s,（ 59. 80 ± 14. 18） %,P〈 0. 05]. Western blot analysis showed that the expression of P2Y4 was reduced in the bladder of the SCI-induced and urine-derived stem cell transplantation group. Conclusion Urine-derived stem cells can promote recovery of neurogenic bladder in rats after SCI.

关 键 词：神经源性膀胱 尿源干细胞 P2Y4 凋亡 

分 类 号：R332[医药卫生—人体生理学] R651.2[医药卫生—基础医学]