NaCl胁迫下交替呼吸途径对烟草悬浮细胞死亡调节作用的研究  

Role of the alternative respiratory pathway in mediating the cell death induced by Na Cl stress on tobacco suspension cells

作　　者：冯汉青[1] 王玉佩[2,3] 王婉[1] 管冬冬[1] 贾凌云[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]中国科学院近代物理研究所,兰州730000 [3]中国科学院大学,北京100049

出　　处：《广西植物》2016年第4期425-429,502,共6页Guihaia

基　　金：国家自然科学基金(31260059;30900105);教育部科学技术研究重点项目(211190);甘肃省财政厅高校科研基本业务费~~

摘　　要：盐分胁迫是植物在自然环境中经常遭遇的环境胁迫因素之一,会引起植物代谢紊乱乃至细胞死亡,这严重限制了植物的生长、繁育和生存。交替呼吸途径是植物较之动物独特的线粒体呼吸途径。该研究在烟草悬浮细胞中调查了交替呼吸途径对Na Cl胁迫引起的植物细胞死亡过程的调节作用及相应的内在机制,以及在200 mmol·L^(-1)Na Cl处理的烟草悬浮细胞中研究了交替呼吸途径和细胞死亡发生及H_2O_2之间的关系。结果表明:(1)随着Na Cl处理浓度的增加,烟草悬浮细胞死亡水平逐渐增加,而交替呼吸途径的容量也逐渐上升。(2)与Na Cl处理相似,外源H_2O_2的处理也能导致烟草悬浮细胞死亡水平的增加。200 mmol·L^(-1)Na Cl的胁迫导致明显的细胞死亡发生和H_2O_2产量的显著性增加;而较之200 mmol·L^(-1)Na Cl胁迫下的细胞,用水杨基氧肟酸(交替呼吸途径的抑制剂)预处理后的细胞再置于200 mmol·L^(-1)Na Cl的胁迫下导致更高水平的细胞死亡和H_2O_2的产生。综上表明,高盐胁迫诱导了烟草悬浮细胞的交替呼吸途径的增加,而交替呼吸途径则可能通过抑制活性氧的产生而起到缓解细胞死亡发生的作用。Soil salinity stress is the one of the most widespread environmental stresses that growing plants have to frequently face. The effects of salinity stress on plants are generally associated with the disruption of metabolism. In particular,high level of salt stress can cause cell death. This seriously limits the development,growth,survival,and productivity of plants. The alternative respiratory pathway is a plant-specific respiratory pathway,which does not exist in animals. By using tobacco suspension cells,the present work investigated whether the alternative respiratory pathway could play a role in mediating the high-salt-induced death of plant cells. And,the possible mechanism for this function of the alternative respiratory pathway was also studied. The results showed that with the increase of the concentrations of Na Cl,the levels of cell death of tobacco suspension cells increased,and the increased levels of cell death were followed with the increases of the capacity of the alternative respiratory pathway. Similar to the treatment with Na Cl,the treatment with exogenous hydrogen peroxide（ H2O2） also induced an increase in the level of cell death. The relationship among the alternative respiratory pathway,cell death,and H2O2 production was studied in the tobacco suspension cell subjected to 200 mmol·L^-1Na Cl. The treatment with 200 mmol · L^-1Na Cl caused an obvious increase of cell death and a significant enhancement of the H2O2 production. The 200 mmol·L^-1Na Cl-stressed cells pretreated with salicylhydroxamic acid,a specific inhibitor of the alternative respiratory pathway,exhibited higher levels of cell death and H2O2 production than the tobacco suspension cells subjected to 200 mmol·L^-1Na Cl stress alone. These results suggest that high salt stress can induce an increase of the alternative respiratory pathway,which plays a role in alleviating cell death under high salt stress. And,this function of the alternative respiratory pathway may relate to its ability to limit the generation of H2O2.

关 键 词：交替呼吸途径 烟草悬浮细胞 H2O2 细胞死亡 

分 类 号：Q945.3[生物学—植物学]