掌叶木种皮障碍及种子各部位内源抑制活性的研究  

Seed coat obstacle and endogenous inhibitory activity of various parts of Handeliodendron bodinieri seed

作　　者：李樱花[1] 郭松[1,2] 李在留[1,3] 李雪萍[4] 李德麟[1] 包满珠[3] 

机构地区：[1]广西大学林学院,南宁530004 [2]南京林业大学江苏省林业生态工程重点实验室,南京210037 [3]华中农业大学园艺林学学院,武汉430070 [4]河南科技大学林学院,河南洛阳471003

出　　处：《广西植物》2016年第4期443-448,470,共7页Guihaia

基　　金：国家自然科学基金(31060053,31560200);广西自然科学基金(2011GXNSFB018016);广西大学大学生实验技能和科技创新能力训练基金(SYJN20131620)

摘　　要：掌叶木(Handeliodendron bodinieri)是残遗于中国的稀有单种属植物,因人为破坏、生境特殊及自身特性的影响,资源稀少,被列为国家一级重点保护野生珍稀濒危植物。该研究以掌叶木种子为材料,研究了4种不同发芽条件下(带种皮、浓硫酸处理种皮、完全去除种皮、仅露出胚根)种子萌发性、种皮透水性、掌叶木果皮、假种皮、种皮和种仁四个部位不同浓度甲醇浸提物(0、3.125、6.25、12.5、25 mg/m L)对白菜种子萌发及幼苗生长的影响以及掌叶木各部位浸提物对种子萌发的影响。结果表明:(1)掌叶木种皮具有一定的透水性,为掌叶木种子的萌发提供必要的透水透气条件,不影响种子萌发前的水分吸收,但掌叶木种皮的机械阻碍、易霉变对种子的萌发影响较大。(2)掌叶木的果皮、假种皮、种皮和种仁甲醇浸提物对白菜种子的萌发和生长都有影响,尤其对白菜幼根的生长有较强的抑制作用,抑制强度依次是种仁>果皮>假种皮>种皮,且随着浓度的升高,抑制作用增强。该研究结果揭示了掌叶木种子难发芽、发芽率低的原因,为掌叶木的人工扩繁和保护与利用奠定了基础。Handeliodendron bodinieri is the relict of rare plant single taxa in China,due to man-made destruction,the special growth environment and the influence of its owe characteristics,with fewer resources,Handeliodendron is listed as national key protection wild treasure and endangered plants. In order to study the effects of rare and endangered H. bodinieri testa on seed germination,through dealing with different conditions（ with the seed coat,concentrated sulfuric acid processed testa,the complete removal of the seed coat,revealing only radicle） of seed germination,seed coat permeable skin and the perivarp,aril,seed coat and kernel of Handeliodendron in different concentrations（ 0,3. 125,6.25,12. 5 and 25 mg / m L） of methanol extracts of planting cabbage seed germination and seedling growth were studied. The results were as follows：（ 1） H. bodinieri tasta had good permeability,water absorption without affecting before seed germination. But testa mechanical obstruction,easy to mold had great impact on seed germination and growth.（ 2） Methanol extracts from fruit shells,arils,seed coats and kernels of H. bodinieri seeds affected the germination and growing of Chinese cabbage seeds and especially greatly affected the growth of radicle. The intensity of inhibition activity was： kernel fruit〉 shell 〉aril〉 seed coat. And the inhibition activity enhanced with the increasing of concentration. The research results revealed the reasons of germination difficulty and the low germination rate and as well to establish the foundation for the artificial propagation and protection and use of H. bodinieri.

关 键 词：掌叶木种子 种皮障碍 透水性 萌发性 发芽抑制活性 

分 类 号：Q945[生物学—植物学]