肾上腺素对布比卡因抑制大鼠心室肌细胞钠电流的影响  

Effect of epinephrine on inhibition of sodium current induced by bupivacaine in ventricular myocytes of rats

作　　者：陈鸿飞[1] 刘付丽[1] 吴一泉[1] 徐旭仲[1] 

机构地区：[1]温州医科大学附属第一医院麻醉科,浙江温州325015

出　　处：《温州医科大学学报》2016年第5期326-329,共4页Journal of Wenzhou Medical University

基　　金：国家自然科学基金资助项目(81470419);浙江省科技厅国际科技合作专项(合作研究)项目(2012C24018);温州市科技局科研基金资助项目(Y20150239)

摘　　要：目的:研究肾上腺素对较低浓度布比卡因所抑制的大鼠心室肌细胞钠电流(I_(Na))的影响。方法:采用急性酶解分离法获得Sprague-Dawley雄性大鼠心室肌细胞,随机分为2组(n=5),以全细胞膜片钳技术记录I_(Na),观察0.15μg/m L肾上腺素对40μmol/L布比卡因和50μmol/L布比卡因所抑制的INa的影响。结果:40μmol/L和50μmol/L布比卡因对大鼠心室肌细胞I_(Na)抑制率分别为(50.2%±5.8%)和(62.7%±7.7%),差异有统计学意义(P<0.05)。当加入0.15μg/m L肾上腺素后,40μmol/L布比卡因组抑制率变到(33.7%±10.2%),差异有统计学意义(P<0.05);50μmol/L布比卡因组抑制率变为(62.1%±7.3%),差异无统计学意义(P>0.05),肾上腺素对I-V曲线无明显影响。结论:肾上腺素可以部分恢复较低浓度布比卡因所抑制的心室肌细胞I_(Na),但其作用在较高浓度布比卡因中受到限制。Objective： To determine the effect of epinephrine on inhibition of sodium current （INa） induced by bupivacaine inventricular myocytes of rats. Methods： The ventricular myocytes isolated from Sprague-Dawley rats by acute enzymatic dissociation were randomly divided into two groups （n=5）. The whole-cell patch clamp technique was used to record INa in single ventricular myocytes and the effect of 0.15 ug/mL epinephrine inhibi- tion of IN, on induced by 40 or 50 μmol/L bupivacaine were observed. Results： The inhibition rates of 40 μmol/L bupivacaine or 50 μmol/L bupivacaine on INa was 50.2%±5.8 % or 62.7%±7.7 % （P〈0.05） respectively. After the administration of 0.15 ug/mL epinephrine, the inhibition rates in the 40 μmol/L bupivacaine group changed to be 33.7%±10.2 % （P〈0.05）, and which was 62.1%±7.3% （P〉0.05） in the 50 μmol/L bupivacaine group. Epinephrine did not shift the I-V curve. Conclusion： Epinephrine can reverse inhibition of INa induced by low-concentration bupivacaine. This effect of epinephrine will be limited to a higher level concentration of bupivacaine.

关 键 词：布比卡因 肾上腺素 钠电流 心室肌细胞 大鼠 

分 类 号：R614[医药卫生—麻醉学]