脑心通对氧化低密度脂蛋白诱导血管平滑肌细胞增殖和自噬的影响  

The effects of naoxintong on vascular smooth muscle cells proliferation and autophagy induced by oxidized low-density lipoprotein

作　　者：邱伟文[1] 郑丽云[1] 邬至平[1] 蔡学礼[1] 彭潇[1] 黄跃金[1] 黄良通[1] 

机构地区：[1]丽水市中心医院神经内科,浙江丽水323000

出　　处：《温州医科大学学报》2016年第5期340-343,347,共5页Journal of Wenzhou Medical University

基　　金：浙江省医药卫生科技项目(2015KYB451)

摘　　要：目的:观察脑心通对氧化低密度脂蛋白(ox-LDL)诱导血管平滑肌细胞(VSMC)增殖和自噬相关蛋白Beclin1、LC3表达的影响,探讨其抗动脉粥样硬化(AS)的可能机制。方法:将人脐动脉VSMC分为:对照组、ox-LDL组、脑心通组、ox-LDL+脑心通组。采用噻唑蓝(MTT)法和克隆形成实验检测细胞增殖作用,Western blot法检测自噬相关蛋白Beclin1与LC3表达水平的改变。结果:ox-LDL显著促进VSMC的增殖和克隆形成,而脑心通对VSMC的增殖和克隆形成无明显作用,但其抑制ox-LDL对VSMC增殖和克隆形成的促进作用。Western blot结果显示ox-LDL显著增加VSMC自噬相关蛋白Beclin1和LC3的表达水平;脑心通抑制oxLDL增加的Beclin1和LC3的表达。MTT法检测发现自噬抑制剂3MA抑制ox-LDL对VSMC的增殖作用。结论:脑心通可能通过降低VSMC自噬抑制ox-LDL对VSMC的增殖作用。Objective： To investigate the effects of naoxintong on the vascular smooth muscle cells （VSMC） proliferation and the expression of Beclinl and LC3 induced by oxidized low-density lipoprotein （ox- LDL）. Methods： The VSMC were divided into four groups： control group, ox-LDL group, naoxintong group and ox-LDL＋naoxintong group. MTT and colony formation assay was used to test the proliferation. Western blot was used to analysis the expression of Beclinl and LC3. Results： ox-LDL significantly promoted the viability and colony formation ofVSMC. Naoxintong had no effect on the proliferation and colony formation but inhibited the proliferation and colony formation of VSMC induced by ox-LDL. Western blot results demonstrated that ox-LDL significantly promoted the expression of Beclil and LC3, naoxintong inhibited the expression of Beclinl and LC3 induced by ox-LDL. Furthermore, MTT verified that autophagy inhibitors 3MA inhibited significantly the proliferation induced by ox-LDL. Conclusion： Naoxintong inhibited the VSMC proliferation induced by ox-LDL through inhibited the cell autophagy.

关 键 词：脑心通 氧化低密度脂蛋白 细胞自噬 血管平滑肌细胞 

分 类 号：R285[医药卫生—中药学] R743.1[医药卫生—中医学]