磁环法快速重建肝上下腔静脉大鼠肝移植模型  

Magnetic ring anastomosis of supra-hepatic vena cava:a novel technique for liver transplantation in rat

作　　者：史源[1] 杨涛[1] 张全胜[1] 张威[1] 刘蕾[1] 邓永林[1] 郑虹[1] 潘澄[1] 沈中阳[1] 

机构地区：[1]天津市器官移植重点实验室,天津市第一中心医院器官移植中心,天津300192

出　　处：《实用器官移植电子杂志》2016年第2期79-82,共4页Practical Journal of Organ Transplantation(Electronic Version)

基　　金：国家高技术研究发展计划(863计划)(2012AA021001);天津市科技计划项目(12ZCZDSY02600);天津市卫生局科技基金(2011KZ30)

摘　　要：目的为改进大鼠肝上下腔静脉(SHVC)重建技术,缩短无肝期时间,提高肝移植存活率,研发微型快速对接磁环。方法 120只雄性Wistar大鼠被随机分为两组,磁环组(n=30),使用磁环进行SHVC重建;缝线组(n=30)使用7-0血管线连续缝合做SHVC重建。两组肝下下腔静脉及门静脉均选择Kamada双袖套法进行重建。结果磁环组大鼠SHVC重建时间、无肝期及手术时间均较缝线组显著减少〔肝上下腔静脉重建时间(分钟):0.91±0.24比10.4±2.11,无肝期时间(分钟):5.63±0.65比17.76±2.51,手术时间(分钟):36.02±8.02比49.38±12.06,均P<0.05〕。磁环组30只受体大鼠中29只存活超过7天,28只存活超过30天;缝线组30只受体大鼠中25只存活时间超过7天,22只存活超过30天(P<0.05)。结论肝上吻合口病理及电镜扫描均提示磁环组肝上吻合口愈合良好。微型磁环快速重建技术,为大鼠肝移植术中肝上下腔静脉重建提供一种新型、简单稳定的方式,明显缩短无肝期,提高手术成功率,降低手术难度。Objective To improve the technique of suprahepatic vena cava（SHVC） reconstruction in rat orthotopic liver transplantation, novel magnetic rings were designed and manufactured to facilitate reconstruction of SHVC and shorten the anhepatic time. Methods 120 adult male Wistar rats were randomly divided into two groups ： rings group（n = 30）, using magnetic rings for SHVC reconstruction; suture group（n = 30）, 7-0 prolene sutures were used for SHVC running anastomosis as control. Cuff techniques were used for portal vein and infrahepatic vena cava reconstruction as Kamada and Calne described. Results Compared with suture group,SHVC reconstruction time, anhepatic phase and recipient operation time were significantly decreased in rings group〔SHVC reconstruction time（min）：0.91±0.24 vs. 10.4±2.11,anhepatic phase time（min）：5.63±0.65 vs. 17.76±2.51,recipient operation time（min）：36.02±8.02 vs. 49.38±12.06,all P〈0.05〕. In the rings group, 29 rats survived for 7 days and 28 rats survived for 30 days. In contrast, only 25 recipients in suture group remained alive for 7 days and 22 survived for more than 30 days（P〈0.05）. Conclusion Better anastomotic healing was founded in rings group by pathology and electron microscope scan. The magnetic rings technique provides a novel, simple method for SHVC reconstruction of orthotopic liver transplantation（OLT） in rat. It significantly shortens anhepatic phase, while the success rate of the operation is satisfactory.

关 键 词：肝移植模型 大鼠 磁压榨 

分 类 号：R657.3[医药卫生—外科学] R-332[医药卫生—临床医学]