胶球藻C-169户外诱导培养积累脂肪酸和色素的研究  

Accumulated Fatty Acids and Pigments in Outdoor Cultured Coccomyxa subellipsoidea C-169

作　　者：魏东[1] 闫文君[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2016年第4期136-140,74,共6页Modern Food Science and Technology

基　　金：国家973科技计划项目(2011CB200904;2011CB200901);国家自然科学基金项目(31370383);国家863科技计划项目(2013AA065802);广东省海洋渔业科技与产业发展专项(A201401C01)

摘　　要：本研究分别采用Basal、BBM以及无氮磷添加的培养基,在户外开放跑道池中培养胶球藻C-169,系统比较了比生长速率、生物量浓度、脂肪酸和色素含量的差异。研究表明,胶球藻C-169在户外开放跑道池中,水温13-20℃下,生长良好。在第二阶段氮磷限制Basal、BBM培养基中的8 d,细胞处于生长的对数期,Basal培养基中平均比生长速率是BBM培养基的1.92倍,生物量干重浓度最高为0.22 g/L。第一阶段细胞在BBM、Basal两种培养基中亚麻酸含量可分别高达总脂肪酸含量的36.6%、53.9%;第二阶段在氮磷限制的BBM培养基中,亚麻酸占总脂肪酸的比例下降了32.4%,油酸的比例增大了38.5%;氮磷限制BBM培养基中细胞内总脂肪酸含量由起始的3.99%增大到18.18%。上述两种藻液以1：1混合培养5 d,平均比生长速率下降到0.0094 d^-1,总脂肪酸含量增大到20.2%。藻细胞中色素含量随着脂肪酸含量的增加而降低,呈明显的负相关。Coccomyxa subellipsoidea C-169 was cultivated outdoors in raceway open ponds using Basal medium,Bold＇s Basal medium（BBM）,and medium without adding nitrogen（N）,and phosphorus（P）.Comparisons were made across the three culture conditions in terms of cell-specific growth rate,biomass concentration,as well as content of fatty acids and pigments.The results showed good growth of Coccomyxa subellipsoidea C-169 at temperatures ranging from 13 ℃ to 20 ℃ in outdoor open ponds.During stage II,cells were in logarithmic phase after eight days of cultivation in Basal medium and BBM with limited N and P,where cell-specific growth rate in Basal medium was 1.92 times that in BBM and maximal biomass was 0.22 g/L.Linolenic acid content in the cells cultured in Basal medium and BBM accounted for 36.6% and 53.9% of total fatty acids,respectively,during stage I.However,during stage II,the proportion of linolenic acid content of total fatty acids in the cells cultured in Basal medium,with N and P limitations decreased by 32.4%,while that of oleic acid increased by 38.5%.In BBM containing low N and P concentrations,the content of total fatty acids increased from 3.99% to 18.18%.In stage III,cells were cultivated for five days in a 1：1 mixture of Basal medium and BBM.During stage Ⅲ,average growth rate of cells decreased to 0.0094 d^-1,total fatty acid content in biomass increased to 20.2% dry weight,and pigment content decreased with increasing fatty acid content,showing a significant negative correlation.

关 键 词：胶球藻C-169 开放式跑道池 氮限制 脂肪酸 叶绿素 

分 类 号：Q946[生物学—植物学] TE667[石油与天然气工程—油气加工工程]