CaM/CaMK Ⅱ在脾气虚证大鼠脑肠微环境中的表达及四君子汤干预效应研究  

Expression of CaM/CaMK Ⅱ and the Intervention effect of Si Jun Zi Decoction in the Brain and Intestine Micro-environment of Rats with Spleen Qi Deficiency Syndrome

作　　者：田茸[1] 巩子汉 杨晓轶[2] 朱立鸣[2] 段永强[2] 成映霞[2] 杜娟[2] 王燕[2] 

机构地区：[1]成都中医药大学,成都611137 [2]甘肃中医学院,兰州730020

出　　处：《中国中医基础医学杂志》2016年第4期468-471,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81160420);甘肃省自然科学基金资助项目(1010RJZA148);甘肃省教育厅基金资助项目(1006-05)

摘　　要：目的:从Ca M信号通路关键基因揭示脾气虚证发生及益气健脾法干预作用机制。方法:脾气虚证大鼠为研究对象,采用实时荧光定量RT-PCR、Western Blot技术检测不同阶段大鼠脑、肠Ca M信号通路关键基因Ca M/Ca MKⅡmRNA和蛋白的动态表达,并分析四君子汤干预效应机制。结果:从大鼠小肠组织看,脾气虚证大鼠相对于正常大鼠Ca M/Ca MKⅡmRNA和蛋白表达升高,经四君子汤治疗后明显降低;从大鼠脑组织看,脾气虚证大鼠相对于正常大鼠Ca M/Ca MKⅡmRNA和蛋白表达降低,经四君子汤治疗后明显升高。结论:脑肠微环境中Ca M信号传导通路关键基因Ca M/Ca MKⅡ的动态表达与脾气虚证形成有关,四君子汤通过影响其表达对脾气虚证起到时相性动态干预。Objective ： To reveal the mechanism about the expressions of key genes of CaM signal pathway of the spleen qi deficiency rats, and the intervention effects about strengthening spleen and benefiting qi. Methods： We took the spleen qi deficiency rats as the targets, We detected dynamic expressions of critical genes CaM/CaMK I1 mRNA and protein on CaM signaling pathway in brain and intestine microenvironments at different time by technique methods of real-time quantitative RT-PCR and Western Blot. At the same time we studied on the intervention effect mechanism of Sijunzi Decoction to treat spleen qi deficiency rats. Results：l Rat intestinal detection index： Spleen qi deficiency rats intestinal CaM/CaMK Ⅱ mRNA and proteins expressions increased compared with normal rats. After the treatment of Sijunzi Decoction rats intestinal CaM/ CaMK Ⅱ mRNA and proteins expressions reduced compared with spleen qi deficiency rats. 2 Rat brain detection index： Spleen qi deficiency rats brain CaM/CaMK Ⅱ mRNA and proteins expressions reduced compared with normal rats. After the treatment of Sijunzi Decoction rats brain CaM/CaMK Ⅱ mRNA and proteins expressions increased compared with spleen qi deficiency rats. Conclusions： The dynamic expressions of key genes CaM/CaMK Ⅱ of CaM signal pathway in brain and intestine microenvironment, which is the basis for spleen qi deficiency syndrome. Sijunzi Decoction treated spleen qi deficiency by affecting the dynamic expressions of CaM/CaMK Ⅱ.

关 键 词：脾气虚证 四君子汤 脑 肠 CAM CaMKⅡ 

分 类 号：R285.5[医药卫生—中药学]