AtAHA10参与拟南芥幼苗盐胁迫响应的信号转导机制初探  

Primary investigation about the signal transduction mechanism of PM H^+-ATPase AHA10 in salt stress response of Arabidopsis thaliana seedlings

作　　者：刘婷[1] 谢世英[1] 王玉芳[1] 尚忠林[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050024

出　　处：《河北农业大学学报》2016年第2期9-14,共6页Journal of Hebei Agricultural University

基　　金：河北省高等学校科学技术研究项目(Q2012065);河北师范大学自然科学科研基金重点基金项目(L2012Z04);河北师范大学自然科学科研基金博士基金项目(L2011B11)资助

摘　　要：为了探究质膜质子泵AHA10在盐胁迫响应中的作用,本试验以拟南芥AHA10缺失突变体aha10-1幼苗为材料,检测了盐胁迫条件下幼苗根和子叶的生长状况。结果显示,aha10-1幼苗在0.1mol/L NaCl处理条件下,主根长度明显短于野生型,子叶生长状况与野生型相同,说明AHA10可能在幼苗根盐胁迫响应过程中发挥了正向调节作用。电生理检测结果显示,盐处理后野生型根细胞质膜钙电流明显增强,而aha10-1根细胞质膜钙电流未见明显变化,表明AHA10可能在盐胁迫激活钙信号过程中发挥了介导作用。Previous studies have revealed that salt stress stimulated the gene expression and activity of H＋-ATPase,and induced cytoplasmic Ca2＋signal in plant cells.But the relationship between the proton pump and cytoplasmic Ca2＋signals under salt stress condition is still unclear.To explore the role of proton pump AHA10 in salt stress response of Arabidopsis seedlings,a null mutant of AHA10（aha10-1）was used as the material in this experiment,and the growths of roots and cotyledons under salt stress were investigated.The results showed that under salt stress,the main root length of aha10-1 after 0.1mol/L NaCl treatment was significantly shorter than that of the wild type（Col-0）,while the cotyledons′growth of aha10-1 was similar to that of the wild type（wt）.The results indicated that AHA10 may play apositive role in salt stress response of roots.Electrophysiological experiment results showed that Ca2＋influx was significantly stimulated by the NaCl treatment in the wild type,but wasimpaired in root protoplasts of aha10-1,indicating that AHA10 may be a key transducer in the process that salt stress stimulated Ca2＋influx.

关 键 词：盐胁迫 拟南芥 质子泵AHA10 钙电流 

分 类 号：Q78[生物学—分子生物学]