不同晶相的草酸钙晶体引起肾上皮细胞的毒性差异  

Toxicity Difference of Calcium Oxalate of Different Crystal Phases on Renal Epithelial Cells

作　　者：孙新园[1] 姚秀琼[1] 余凯[1] 欧阳健明[1] 

机构地区：[1]暨南大学生物矿化与结石病防治研究所,广州510632

出　　处：《无机化学学报》2016年第5期818-826,共9页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21371077)资助项目

摘　　要：采用扫描电子显微镜(SEM)、激光共聚焦显微镜(LSM)、流式细胞仪、电感耦合等离子体发射光谱仪(ICP)等方法比较研究了尺寸约1.2μm的一水草酸钙(COM)和二水草酸钙(COD)晶体对非洲绿猴肾上皮细胞(Vero细胞)的损伤差异。COM和COD均能引起Vero细胞的细胞活力和超氧化物歧化酶(SOD)活力降低、丙二醛(MDA)含量升高以及碘化丙啶(PI)红染细胞数目增多,且均呈现浓度依赖性。在相同晶体浓度时,COM的细胞毒性明显比COD强,引起细胞表面表达的粘附分子透明质酸(HA)比COD多,粘附量也显著增加。相比于COM晶体,Vero细胞更容易内吞COD晶体。COM与COD晶体对肾上皮细胞的毒性差异与晶面的离子密度以及细胞与晶体间的静电作用等因素密切相关。本文结果将有助于从分子水平上和细胞水平上阐释草酸钙结石的形成机理。Scanning electron microscope （SEM）, laser confocal microscope （LSM）, flow cytometry, inductively coupled plasma emission spectrometer （ICP） methods were used to comparatively study the cell injury differences on the African green monkey kidney epithelial （Vero） cells by calcium oxalate monohydrate （COM） and dihydrate （COD） crystals with a size about 1.2 μm. Both COM and COD could decrease the cell viability and superoxide dismutase （SOD） activity, increase malondialdehyde （MDA） content and the number of red dye cells staining with propidium iodide （PI） in a dose-dependent manners. Compared to COD crystals, COM crystals induced significantly higher toxicity under the same crystal concentration, and COM crystals caused more adhesion molecules hyaluronic acid （HA） expression and higher adhesion amount than COD crystals. The ability of the Vero cells to endocytose COD crystal was stronger than that of COM. The cytotoxicity difference between COM and COD was closely related to ion density of crystal face and electrostatic interactions between the cell and crystals. The results in this paper can heXp to explain the formation mechanism of calcium oxalate stones at the molecular level and cellular level.

关 键 词：一水草酸钙 二水草酸钙 细胞毒性 浓度效应 

分 类 号：R69[医药卫生—泌尿科学] R329.28[医药卫生—外科学]