跌打万花巴布剂的经皮渗透研究  

Experimental study on transdermal characteristics in vitro of Diedawanhua patches

作　　者：张鹰[1] 刘新国[1] 熊鑫[1] 程璐[1] 张红[1] 

机构地区：[1]武汉市第一医院,湖北武汉430022

出　　处：《中国医院药学杂志》2016年第9期723-727,共5页Chinese Journal of Hospital Pharmacy

基　　金：武汉市卫生局临床医学科研项目(编号:WZ14Z07)

摘　　要：目的：研究跌打万花巴布剂体外经皮渗透特征。方法：采用Franz扩散池和离体大鼠皮肤进行体外渗透实验,利用高效液相色谱法测定蛇床子素、水杨酸甲酯和丁香酚的累积透皮量。色谱柱：Phenomenex Hyperclone BDS C18柱（250×4.00mm,5μm）;流动相：甲醇-水-冰醋酸（60∶40∶0.25）;流速：1.0mL·min^-1;检测波长：322,270,290nm;进样量：10μL;柱温：室温。结果：当基质载药量≤3%时,蛇床子素的体外经皮渗透符合零级动力学方程,但透皮速率的增长随载药量的增加而明显降低。对于水杨酸甲酯和丁香酚成分,当载药量在2.0%-8%范围内时,其体外经皮渗透行为均符合Higuchi方程,透皮速率的增长趋势并未随载药量的增加而明显降低。结论：跌打万花巴布剂载药量对指标成分的经皮渗透行为影响显著,而皮肤限速作用对各成分的影响又各不相同,可参考经皮渗透实验结果优化中药巴布剂基质载药量和药味配比。OBJECTIVE To study the transdermal characteristics of Diedawanhua pathes in vitro.METHODS The permeation of Diedawanhua patches was studied with Franz diffusing cell and mice skin.The cumulative percent releasing amount of osthol,methyl salicylate and eugenol was determined by HPLC.The column：Phenomenex Hyperclone BDS C18column（250×4.00 mm,5μm）;The mobile phase∶Methanol∶water∶glacial acetic acid=60∶40∶0.25;The flow rate：1.0mL·min^-1;The detection wavelength：270,290,322 nm;sample volume：10μL; The column temperature is indoor temperature.RESULTS When the matrix loading is less than or equal to 3%,transdermal permeation of Osthol in vitro accords with zero order kinetic equation.But the increase of the penetration rate was significantly decreased with the increase of drug loading.In the range of 2%to 8%,Methyl salicylate and Eugenol permeation behavior are coincident with higuchi equation.The growth trend of skin penetration rate was not significantly decreased with the increase of drug loading.CONCLUSION The effect of drug loading on the transdermal penetration of the index components was significant,and skin played different speed limiting effect on different composition,so percutaneous penetration experiment results should be consulted to optimize the loading and proportion of the drug.

关 键 词：跌打万花巴布剂 蛇床子素 丁香酚 水杨酸甲酯 经皮渗透 高效液相色谱法 

分 类 号：R943[医药卫生—药剂学]