枫蓼提取物对小鼠实验性溃疡性结肠炎的防治作用  

Effect of Fengliao extract on mice with experimental ulcerative colitis

作　　者：任守忠[1] 陈君[2] 苏文琴[1] 王宁[1] 马志健[3] 

机构地区：[1]海南医学院药学院,海南海口571199 [2]海南省人民医院药学部,海南海口571017 [3]海南医学院解剖教研室,海南海口571199

出　　处：《中国药理学与毒理学杂志》2016年第4期344-349,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金(81441137);海南医学院引进人才科研启动经费资助~~

摘　　要：目的探讨枫蓼提取物对小鼠溃疡性结肠炎的防治作用。方法通过小肠推进实验和番泻叶致小鼠腹泻模型观察小肠推进率、稀便率及腹泻指数。用4%葡聚糖硫酸钠(DSS)建立小鼠溃疡性结肠炎模型,对正常组、模型组、美沙拉嗪组、枫蓼提取物11.7,23.4和46.8 g·kg-1剂量组小鼠进行疾病活动指数(DAI)评分,测定结肠组织中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、丙二醛(MDA)和一氧化氮(NO)含量及髓过氧化物酶(MPO)的活性。结果枫蓼提取物46.8 g·kg-1能抑制小鼠小肠推进率,显著减少番泻叶致腹泻小鼠腹泻次数,降低腹泻率和腹泻指数(P<0.05)。结肠炎结果显示,与正常组比较,模型组小鼠体质量减轻,DAI评分增高(P<0.05),结肠组织中MPO活性、IL-1β、TNF-α、MDA和NO含量升高(P<0.01)。与模型组比较,枫蓼提取物46.8 g·kg-1组小鼠DAI评分降低29.1%(P<0.05);23.4和46.8 g·kg-1组结肠组织中MPO活性、TNF-α、MDA和NO含量均降低(P<0.05)。结论枫蓼提取物能改善DSS诱导小鼠溃疡性结肠炎,可能与解痉、止泻、抗炎、抗氧化及减少炎性介质释放等有关。OJECTIVE To investigate the preventive effect of Fengliao extract on ulcerative colitis of mice. METHODS Using the intestinal propulsion rate experiment and senna induced diarrhea model,the intestinal propulsion rate, diarrhea rate and index of diarrhea were observed. Mice were randomly divided into normal group,model group,mesalazine hydrochloride group and Fengliao extract 11.7,23.4 and 46.8 g·kg- 1group. The mouse colitis model was induced by 4% dextran sulfate sodium. The mice were administraed once daily for 7 d while the disease activity index（DAI）score was calculated and the activity of tumor necrosis factor α（TNF-α）,interleukin-1β（IL-1β）, myeloperoxidase（MPO）and content of malondialdehyde（MDA） and nitric monoxide（NO）in colon tissue were determined.RESULTS Fengliao extract 46.8 g·kg- 1inhibited the intestinal propulsion rate（P〈0.05）,reduced the frequency of diarrhea and the diarrhea index（P〈0.05）. Results of colitis showed that the body mass of mice in the model group was significantly decreased but the DAI score increased compared with normal group（P〈0.05）. The activity of MPO and the contents of IL-1β,TNF-α,MDA and NO in colon mucosa were increased（P〈0.01）. Compared with the model group,Fengliao extract 46.8 g·kg-1decreased the DAI score（P〈0.05）while Fengliao extract 46.8 and 23.4 g·kg-1reduced MPO activity in colonic mucosa and content of IL- 1β,TNF- α,MDA and NO in colonic homogenate（P〈0.05）. CONCLUSION Fengliao extract can significantly improve the DSS induced colitis in mice,which is probably associated with its antispasmodic and anti-diarrheal effect as well as the reduced release of inflammatory mediators and antioxidants.

关 键 词：枫蓼提取物 结肠炎 溃疡性 

分 类 号：R285.5[医药卫生—中药学]