不同日龄苏太仔猪FUT2基因的组织表达谱和表达差异分析  

Tissue Expression Profile and Differential Expression of FUT2 Gene in Sutai Piglets at Different Ages

作　　者：夏日炜 钦伟云 甘丽娜[1] 包文斌[1,2] 吴圣龙[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [2]江苏省种猪繁育和健康养殖工程技术研究中心,江苏扬州225009

出　　处：《中国畜牧杂志》2016年第9期1-4,共4页Chinese Journal of Animal Science

基　　金：江苏省科技支撑计划(BE2015329,BE2014357);江苏省农业三项工程[SXGC(2015)326]

摘　　要：目前有研究表明FUT2基因是仔猪大肠杆菌F18受体形成的关键候选基因,探讨FUT2基因在仔猪出生至断奶期间的m RNA表达变化,可为进一步研究该基因对F18大肠杆菌致病的相关性提供一定的理论依据。本试验挑选4个日龄(8、18、30日龄和35日龄)苏太仔猪各4头,通过利用Real-time PCR方法分别比较分析了FUT2基因在各个体11个组织(心、肝、脾、肺、肾、胃、肌肉、胸腺、淋巴结、十二指肠、空肠)中的表达规律。结果表明:FUT2基因在不同日龄苏太猪11个组织中均有表达,其中在肺、肾、胃、胸腺、淋巴和十二指肠中呈现较高表达,在肝、脾、空肠中呈现中度表达,在心和肌肉中呈现低表达。FUT2基因在8日龄个体空肠组织上的表达水平显著的高于其他3个日龄(P<0.05),而不同日龄仔猪十二指肠中的FUT2基因表达水平间没有显著差异(P>0.05)。由此推测,8日龄左右FUT2基因的高表达可能有利于仔猪空肠组织中F18大肠杆菌受体的快速形成,需进一步对仔猪8日龄至18日龄间FUT2基因的表达变化做进一步细致的检测。Previous studies have showed that FUT2 gene is one of the key candidate genes for formation of E. coli F18 receptor in piglets. Analysis of FUT2 gene mRNA expression from birth to weaning piglets can provide theory basis for further studying on the correlation between this gene and pathogenicity of E. coli F18. In this experiment, four Sutai piglets from different ages （8, 18, 30 and 35 day-old） respectively were used and expression patterns of FUT2 gene in 11 tissues （heart, liver, spleen, lung, kidney, stomach, muscle, thymus, lymph node, duodenum, jejunmn） of each individual weredetermined by Real-time PCR. The results revealed that the FUT2 expression levels were higher in the lung, kidney, stomach, thymus, lymph node and duodenum, medium in the liver, spleen and jejunum and lower in the heart and muscle. The FUT2 expression in jejunum of 8 day-old individuals was higher than that of other age piglets significantly （P 〈0.05）, but the expression in duodenum had no significant difference （P〉0.05）. The results speculated that the higher FUT2 expression mightcontribut to the rapid formation of E. coli F18 receptor in intestinal tissue of piglets around 8 day-old, therefore the FUT2 expression between 8 and 18 day-old needed to be further detected carefully.

关 键 词：猪 FUT2基因 F18大肠杆菌 MRNA表达 

分 类 号：S828.2[农业科学—畜牧学]