非诺贝特对严重烧伤大鼠肝脏脂肪变性的疗效观察  

Efficacy of fenofibrate for hepatic steatosis in rats after severe burn

作　　者：黄宗伟[1] 蒙诚跃[1] 陈婧[2] 陈雅洁[2] 陈渝[2] 周涛[2] 阳超[1] 

机构地区：[1]广西医科大学第一附属医院烧伤整形科,南宁530021 [2]第三军医大学西南医院全军烧伤研究所 创伤、烧伤与复合伤国家重点实验室

出　　处：《中华烧伤杂志》2016年第5期277-282,共6页Chinese Journal of Burns

基　　金：广西壮族自治区教育厅高校科研项目（YB2014074）

摘　　要：目的观察非诺贝特对严重烧伤大鼠肝脏脂肪变性的疗效。方法将27只雄性sD大鼠按照随机数字表法分为假伤组、单纯烧伤组、烧伤＋非诺贝特组，每组9只。假伤组大鼠将背部浸入37℃温水中15s模拟致伤，之后不予处理。其余2组大鼠将背部浸入98℃热水中15s造成30％TBSAm度烫伤（以下称烧伤），伤后1h腹腔注射乳酸钠林格液。伤后24h起，烧伤＋非诺贝特组大鼠给予生理盐水稀释的非诺贝特（药物剂量为80mg·kg-1·d-1），连续给药7d，单纯烧伤组大鼠则给予等体积生理盐水。（1）于伤后4、6、8d，各组分别选取3只大鼠采集下腔静脉血，采用全自动生化分析仪检测血清总胆固醇（TC）、甘油三酯（TG）、游离脂肪酸（FFA）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）含量；取血后称取大鼠体质量，随即处死大鼠取肝脏称取湿质量，计算后者与前者比值即肝脏指数，同时行肝脏组织大体观察。（2）各时相点下每只大鼠留取1块肝脏组织样本，HE染色观察组织病理学变化；各时相点下每只大鼠选取1张HE染色切片，评估肝脏脂肪变性程度，并统计各组各级肝脏脂肪变性鼠数。对计量资料行析因设计方差分析及SNK检验，对计数资料行Kruskal-Wallis检验和Nemenyi检验。结果（1）伤后4d，单纯烧伤组大鼠TC、TG、FFA、HDL含量与假伤组比较，差异明显（P值均小于0．05）；烧伤＋非诺贝特组大鼠TC、TG、FFA含量均明显低于单纯烧伤组（P值均小于0．05），HDL含量与单纯烧伤组相近（P〉0．05）；3组大鼠LDL含量相近（P值均大于0．05）。伤后6d，单纯烧伤组大鼠TC、TG、HDL含量与假伤组比较，差异明显（P每均小于0．05）；烧伤＋非诺贝特组大鼠TC、TG含量均明显低于单纯烧伤组（P值均小于0．05），HDL含量明显高于单纯烧伤组（P〈0．05）；3组大鼠FFA、LDL含量相近（P值均大�Objective To observe the efficacy of fenofibrate for hepatic steatosis in rats after severe burn. Methods Twenty-seven male SD rats were divided into sham injury group, burn group, and burn ＋ fenofibrate group according to the random number table, with 9 rats in each group. Rats in sham injury group were sham injured on the back by immersing in 37 ℃ warm water for 15 s and then remained without other treatment. Rats in burn group and burn ＋ fenofibrate group were inflicted with 30% total body surface area full-thickness scald （hereinafter referred to as burn） on the back by immersing in 98 ℃ hot water for 15 s, and then they were intraperitoneally injected with lactated Ringer＇s solution at post injury hour （PIH） 1. From PIH 24 to post injury day （PID） 8, rats in burn ＋ fenofibrate group were treated with fenofibrate in the dose of 80 mg· kg-1· d-1 , while those in burn group were treated with equivalent volume of saline. （1） Three rats of each group were respectively selected on PID 4, 6, and 8 for the collection of inferior vena ea- val blood samples. Serum content of total cholesterol （TC）, triglyceride （TG） , free fatty acid （FFA） , high density lipoprotein （HDL）, and low density lipoprotein （LDL） was determined with fully automatic bio- chemical analyzer. Body mass of each rat was measured immediately after blood sampling, and then rats were sacrificed to collect liver tissue for weighing wet mass, The ratio of wet mass of liver tissue to body mass （liver index） was calculated. Meanwhile, gross observation of liver was performed. （2） One liver tissue sample was harvested from each rat at each time point to observe histopathologic changes with HE staining. One liver tissue slice of each rat at each time point was collected to evaluate degree of hepatic steatosis, and the number of rats in each group in each grade of hepatic steatosis was recorded. Measurement data were pro- cessed with analysis of variance of factorial design and SNK test, and enu

关 键 词：烧伤 肝细胞 非诺贝特 血脂异常 脂肪变性 

分 类 号：R644[医药卫生—外科学]