白细胞介素18基因多态性与HBV所致肝细胞肝癌的相关性  

Association between interleukin-18 gene polymorphisms and hepatocellular carcinoma caused by hepatitis B virus

作　　者：张清秀[1] 姚云清[1] 李始亮 龙琴[1] 

机构地区：[1]重庆医科大学附属第一医院感染科,400016

出　　处：《中华肝脏病杂志》2016年第5期352-357,共6页Chinese Journal of Hepatology

基　　金：重庆市医学科研计划重点项目（2015ZDXM008）;重庆市自然科学基金项目（20021889）

摘　　要：目的探讨白细胞介素18（IL-18）基因-137G／c（rS187238）和-607A／C（rs1946518）位点单核苷酸多态性（SNP）与HBV所致肝细胞性肝癌（HCC）的相关I生。方法研究对象分3组：HBV相关性HCC组109例，慢性HBV感染组113例，健康对照组127例。采用聚合酶链反应-连接酶反应（PCR-LDR）基因分型技术检测各组IL-18基因-137G／C和-607A／c两个位点等位基因及基因型。对基线资料采用t检验、x2检验；分析各基因型、等位基因频率在各组中分布是否有差异采用x2检验；比较各组基因型、等位基因与HBV相关性HCC发病风险的OR值、95％可信区间（CI）采用非条件logistic回归分析。结果IL-18-607A／C位点AA基因型及A等位基因在HBV相关性HCC组的分布步页率（29．4％、54．6％）均显著高于健康对照组（18．1％、44．1％，x2=4．152，P〈0．05；X2=5．169，P〈0．05），与HBV相关性HCC罹患风险呈正相关（OR=1．879，95％a：1．020～3．464；OR=1．524，95％CI：1．059～2．193）。IL-18-607A／C位点A等位基因在慢性HBV感染组的分布步页率（54．0％）显著高于健康对照组（44．1％）（X2=4．680，JD〈0．05），与幔性HBV感染风险呈正相关（伽=1．487，95％a：1．037～2．132）。IL-18-607A／C位点基因型及等位基因在HBV相关性HCC组与慢性HBV感染组间的分布频率没有明显差异（P〉0．05）。IL-18-137G／C位点基因型与等位基因分布频率在三组间两两比较，结果均无明显差异（P〉0．05）。结论IL-18-607A／C位点AA基因型及A等位基因与HBV相关性HCC罹患率呈正相关，IL-18-607A／C位点A等位基因与陧性HBV感染罹患率呈正相关。Objective To investigate the association between the single nucleotide polymorphisms （SNPs） IL-18-137G/C （rs187238） and IL-18-607A/C （rs1946518） in interleukin-18 （IL-18） gene and hepatocellular carcinoma （HCC） caused by the hepatitis B virus （HBV）. Methods The subjects were divided into HBV- related HCC group （109 patients）, chronic HBV infection group （113 patients）, and healthy control group （127 patients）. The polymerase chain reaction-ligase detection reaction （PCR-LDR） was used to determine the alleles and genotypes of the two SNPs IL-18-137G/C and IL-18-607A/C. The t-test and chi-square test were used for baseline data. The chi-square test was used to investigate the differences in genotype and allele frequencies across the three groups. Non-conditional logistic regression analysis was used to compare the odds ratios （ORs） and 95% confidence intervals （Cls） for different genotypes/alleles in predicting the risk ofHBV-related HCC. Results The HBV-related HCC group showed significantly higher AA genotype and A allele frequencies of the SNP IL- l 8- 607A/C than the healthy control group （AA genotype frequency： 29.4% vs 18.1%,Z 2 = 4.152, P 〈 0.05; A allele frequency： 54.6% vs 44.1%, 5.169, P 〈 0.05）, which were positively correlated with the risk of HBV-related HCC （AA genotype frequency： OR = 1.879, 95% CI： 1.020-3.464; A allele frequency： OR = 1.524, 95% CI： 1.059- 2.193）. The chronic HBV infection group had a significantly higher A allele frequency of the SNP IL-18-607A/ C than the healthy control group （54.0% vs 44.1%,Z 2 = 4.680, P 〈 0.05）, which was positively correlated with the risk of chronic HBV infection （OR = 1.487, 95% CI： 1.037-2.132）. The genotype and allele frequencies of the SNP IL-18-607A/C showed no significant differences between the HBV-related HCC group and the chronic HBV infection group （P 〉 0.05）. The genotype and allele frequencies of the SNP IL-18-137G/C showed no significant differences betwee

关 键 词：癌 肝细胞 肝炎病毒 乙型 白细胞介素18 单核苷酸多态性 

分 类 号：R512.62[医药卫生—内科学]