空间诱变水稻品系T2的稻瘟病抗性分析及抗病基因定位  

Analysis of Resistance to Blast Isolates and Mapping of Rice Blast Resistance Gene in T2

作　　者：孙大元[1] 陈冠州[1] 张景欣[1] 周丹华[2] 王慧[2] 朱小源[1] 杨祁云[1] 陈志强[2] 

机构地区：[1]广东省农业科学院植物保护研究所广东省植物保护新技术重点实验室,广东广州510640 [2]华南农业大学国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《华北农学报》2016年第2期7-11,共5页Acta Agriculturae Boreali-Sinica

基　　金："十二五"国家"863"计划项目(2012AA101201)

摘　　要：为了弄清水稻空间诱变稻瘟病抗性变异遗传机理,以一个空间诱变抗稻瘟病突变体T2为研究对象,采取抗谱测定,遗传分析及基因定位等方法,结果发现:2013年晚造T2抗谱达到90.6%,高于部分广东主栽品种;遗传分析表明,其对GD0193的抗性由1个显性主效基因控制,暂命名为PiTH;并通过SSR和InDel标记将该基因定位于水稻11号染色体SSR标记T5与InDel标记K10之间约1.63cM的遗传区域内。目前,该位点包含Pik抗病基因簇,因抗性差异,PiTH可能为该位点一新基因。To understand the genetic mechanisms of space-induced rice mutants＇ resistance to blast,T2,an indica rice mutant from Taihang 68,was researched in this study. Resistant spectrum,genetic analysis and gene mapping were applied in this study. It was found that T2 conferred broader resistance spectrum than most of main cultivars from Guangdong Province. By genetic analysis,the R gene was controlled by a single dominant gene,temporarily designed as PiTH gene,which was further mapped between T5 and K10 on chromosome 11 using SSR and InDel markers. PiTH might be a novel resistance gene in the Pik cluster,which conditioned differential reactions against many isolates and contained higher resistance.

关 键 词：水稻 稻瘟病 抗病基因 基因定位 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]